2010 ECIS Users Meeting
The ECIS Users meeting, held August 24-27 at the historic
Rensselaerville Institute in Upstate NY, was a great success both
scientifically and socially. Over 40 attendees from the United States and
Europe met for three days of talks, poster sessions and workshops. Research
topics varied, but all had the common theme of employing ECIS measurements to
discern changes in cell behavior. It is difficult to single out any particular
talk, but one that stimulated much interest was the first description of the
use of ECIS to follow adipose-derived stem cell differentiation.
On Wednesday evening, Ivar Giaever, CTO of Applied
BioPhysics and recipient of the 1973 Nobel Prize in physics, gave a stimulating
and humorous talk entitled "How to Win a Nobel Prize." The Rensselaerville
Institute staff provided delicious meals throughout the meeting including a
wonderful Thursday night barbecue held outdoors under starry skies. In
addition, each night there were fireside discussion sessions where ECIS users
exchanged ideas, shared common experiences and formed new friendships.
We look forward to next year where tentative plans are in
place for another ECIS Users Meeting in Regensburg, Germany in late July 2011. Stay
tuned for details.
To view pictures of the meeting please visit our Facebook Page.
New ECIS Arrays
(Cell Proliferation) -- Each of the 8 wells has two sets of 7.5mm
x 0.5mm rectangular active electrodes located on inter-digitated fingers to
provide measurements of cells. Each well
has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. On
average, with a confluent layer, approximately 4000 to 8000 cells will be
measured by the electrodes.
The 8WCP arrays are designed
to monitor larger numbers of cells, sampling over the entire bottom of the
well. Because of the relatively high number of cells, impedance fluctuations
due to micromotion are smoothed out and do not obscure subtle changes in
impedance due to the experimental conditions.
Recommended for use with Cell
(Wounding) -- Each of the 8 wells contains a single linear electrode
with dimensions of 667µm x 150µm and an area equal to that of our standard
250µm circular electrodes. Each well has a substrate area of 0.8 cm2
and a maximum volume of 600μL. On average, with a confluent cell layer,
approximately 50 to 100 cells will be measured by the electrode, but even a
single cell can be observed.
Recommended for use with cell
migration measurements via automated wound healing.
2W4x10E -- This array configuration was developed in
collaboration with Yang Wang of the University of Chicago. Dr. Wang's
requirements were for arrays with a large surface area to volume ratio to allow
for rapid gas exchange within the media. These arrays have greatly aided Dr.
Wang in his ECIS anoxia experiments. Each of the 2 circular 25 mm diameter
wells contain four independent sets of ten 250 μm diameter active
electrodes. In addition, the center of these arrays are left clear,
without gold or photo-resist allowing for live cell Fluorescent microscopy with
a standard inverted microscope.
8F10E -- This is a
specialized array with 8 sets of 10 active 250 μm diameter electrodes located in
the central region at the base of a flow channel measuring 50 mm long, 5 mm in
width and 0.4 mm in height.
The flow array is useful for
ECIS measurements of cells under perfused conditions or to mimic the shear
stress endothelial cells experience in vivo. The channel has an area of
2.5 cm2 and a channel volume of 100 μL. On average, a confluent
layer of approximately 500 to 1000 cells will be monitored by each set of electrodes.
HNA Arrays -- We can
customize any of our 8 well arrays with a thin substrate, the thickness of a
coverslip. Recommended for use with High
Numerical Aperture Microscopy.
ABP Beta Tests a New Wounding Method: "The Electric Fence"
The Electric Fence is an experimental alternative
method to the conventional ECIS wound healing protocol. Instead of growing
cells to confluence and then wounding the cells with high current, high current
is used to prevent cells from growing on the electrode surface. Cells grow on
all areas of the well bottom except the "fenced" electrode.
Once a confluent layer has been established
around the electrode, the "electric fence" is turned off, and cells migrate
inward to fill the open area. This population of the electrode is monitored
with normal ECIS measurements, and, as in the wounding assay, cells must move
the radius of the electrode to bring the final impedance values up to those of
the control cell-covered electrodes.
This alternative method is particularly useful in cases where standard
ECIS wounding does not work well because cells do not easily release after
wounding. ABP is currently beta testing the Electric Fence with
select customers. It uses the current ECIS wounding hardware and requires
only a change to the software. If you are also interested in acting
as a beta tester, please contact Christian Renken at firstname.lastname@example.org.
CO2 Microscope Stage Incubator System The
Model S1S ECIS stage incubator system is a turn-key system to provide proper
temperature and atmospheric conditions to allow simultaneous ECIS and optical
measurements. The device fits on the stage of an inverted tissue culture
microscope and accommodates up to two 8 well ECIS arrays. Temperature,
monitored by a thermocouple located in the chamber, is controlled by a
circulating water bath connected to the device. Temperature output can be
logged with the ECIS data. Two gas flow rotameters and a bubble humidifier are
included to control the atmosphere within the incubator. The system includes
all necessary tubing and fittings.For more information visit: Stage Incubator
The ECIS Environmental Control
Cabinet allows researchers to monitor cell behavior and conduct ECIS
experiments in a controlled gas environment.The cabinet will accept either the
16 or 96 well ECIS Station and fits within a standard tissue culture incubator
that is used to regulate the cabinet's temperature. Optional gas sensors within
the cabinet are used to measure and regulate the concentration of oxygen and/or
carbon dioxide within the cabinet. Pull-out shelves provide easy access to the
array holder. Cabinet size: 8.25" H x 16" W x 14"
DOverall footprint: 13" H x 16" W x
15" D For more information visit: Hypoxia Chamber
ECIS Early Career Grant
Applied BioPhysics would like to help young scientists
obtain funding. The ECIS mini-grant is aimed at early career scientists
who are applying for their first RO1 grant. For a researcher wanting
to use ECIS technology to achieve their research goals, Applied
BioPhysics will provide an ECIS instrument, ECIS arrays, and
consultation in order to generate preliminary data to support the
applicants RO1 proposal. Interested scientists should submit their
research plan with a cover letter explaining how ECIS technology can be
used to achieve their specific objectives. Applied BioPhysics will
evaluate proposals based on scientific merit, suitability with ECIS
technology and novelty.
To apply please send a resume, RO1 research plan and cover letter to Christian Renken at email@example.com.
The latest version of
ECIS software is v1.2.55 (for the PC).
1. When using the
software you may notice a black console window in the background. This is
normal and the window must not be closed, but can be minimized. Any messages
from the software will appear in the console window and can be reported to ABP in case of
2. When acquiring
data the GUI (graphical user interface) will be partially locked.
This can be overridden by
un-checking the 'lock' icon on the toolbar. The GUI lock will disable all menu items, the
ability to change datasets or switch to Analysis. Only certain toolbar buttons will be available; zoom, pan, data cursor, legend, full screen and thumbnails are all disabled.
To minimize potential Windows issues during acquisition the following steps are
A number of improvements are the result of suggestions received from the recent ECIS users
off Wifi and disconnect from the internet
any Bluetooth devices and stop any Bluetooth services or taskbar
the webcam and any Bluetooth devices from the BIOS
all other applications
sure any system updates have been installed so Windows doesn't force an
any unnecessary software for applications that appear on the taskbar
at least 1GB of disk space is available
a fresh copy of the ECIS Software prior to starting acquisition
all USB cables are firmly attached
the Power Management profile is 'Always On'
of Error Bar data when exporting Graph Data (File | Export | Graph Data)
color palettes for 8 well arrays (Edit | Color Palette)
Download and install
the update from: If your system is on
the internet, go to 'Help | Check for Updates' in the software.
You can also install
ECIS software on different computers for offline analysis using the CD that
came with your system.
You can also download
the base files from:
|ECIS Webinar Schedule 2010
ECIS application webinars review the topics listed below in 20 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese. https://appliedbiophysics.webex.com
All webinars are held at 11:00am EST. To register for a webinar, please go to:
For a more detailed description of each webinar, please visit: http://www.appliedbiophysics.com/contactUs/webinar.html.
Automated Cell Migration - September 21, 2010
Barrier Function Assays - October 5, 2010
Real-time Electroporation and Monitoring - October
Cell Attachment and Spreading Measurements - November
Signal Transduction Assays - November 16, 2010
Toxicology with ECIS - December 7, 2010
of Matrix-Dependent Cell Migration with a Barrier Migration Assay
Sven Kroening and Margarete Goppelt-Struebe. Sci.
Signal. 2010; 3:pl1.
Recruits the Adapter Protein CMS/CD2AP to Cell-Cell Contacts. Trynette J. van
Duijn, Eloise C. Anthony, Paul J. Hensbergen, André M. Deelder, and Peter L.
Hordijk. J. Biol. Chem.
regulates endothelial permeability and neutrophil migration via Src/ERK1/2
Sun, Mack H. Wu, Mingzhang Guo, Mark L. Day, Eugene S. Lee, and Sarah Y. Yuan. Cardiovasc Res. 2010;
induces lymphangiogenesis in vivo. Frederic Larrieu-Lahargue, Alana L. Welm,
Kirk R. Thomas, and Dean Y. Li. Blood.
Protein Kinase II Delta 6 (CaMKII6) and RhoA Involvement in
Thrombin-induced Endothelial Barrier Dysfunction. Zhen Wang, Roman
Ginnan, Iskandar F. Abdullaev, Mohamed Trebak, Peter A. Vincent, and Harold A.
Singer. J. Biol. Chem.
regulates re-annealing of endothelial adherens junctions through
transcriptional control of β-catenin expression. Muhammad K. Mirza,
Ying Sun, Yidan D. Zhao, Hari-Hara S.K. Potula, Randall S. Frey, Steven M.
Vogel, Asrar B. Malik, and You-Yang Zhao. J.
Exp. Med. published 26 July 2010, 10.1084/jem.20091857.
of negative pressures on epithelial tight junctions and migration in wound
Hsu, Wen-Chung Tsai, Carl Pai-Chu Chen, Yun-Mei Lu, and Jong-Shyan Wang. Am J Physiol Cell Physiol.
Stem Cell Treatment in Mice Attenuates Lung and Systemic Injury Induced by
Cigarette Smoking. Kelly Schweitzer, Brian H Johnstone, Jana Garrison,
Natalia Rush, Scott Cooper, Dmitry O Traktuev, Dongni Feng, Jeremy J Adamowicz,
Mary Van Demark, Amanda J Fisher, Krzysztof Kamocki, Mary Beth Brown, Robert G
Presson, Jr, Hal E Broxmeyer, Keith L March, and Irina Petrache. Am. J. Respir. Crit. Care Med.
published 13 August 2010, 10.1164/rccm.201001-0126OC
efficient analysis of nanomaterial cytotoxicity based on bioimpedance. K Kandasamy, CS Choi, and S Kim. Nanotechnology.
2010; 21: 375501.
regulates human angiotensin II type 1 receptor expression in intestinal
epithelial C2BBe1 cells. Sarah E. Sansom, Gerard J. Nuovo, Mickey M.
Martin, Sainath R. Kotha, Narasimham L. Parinandi, and Terry S. Elton. Am J Physiol Gastrointest Liver
Physiol. 2010; 299:G632-G642.
suppressor function of MDA-7 / IL-24 in human breast cancer. N Patani, A
Douglas-Jones, R Mansel, W Jiang, and K Mokbel. Cancer Cell Int. 2010; 10:29.
Have you recently published an article that includes the use of ECIS? If so, submit your publications to Applied BioPhysics via email to Nancy Vlahos at firstname.lastname@example.org. We
will announce your article in our newsletter, post it on our website and send you 2
FREE 8 well arrays!
|Visit Us at Upcoming Events
Applied BioPhysics will have ECIS demonstrations and informational
literature at the following tradeshows and events:
American Society for Cell Biology
ASCB 50th Annual Meeting
December 11-15, 2010
|Tip of the Month: |
Improving Experiment Repeatability with Cysteine
electrical stabilization (see June 09 Newsletter) or cysteine treatment
is strongly recommended to enhance experimental repeatability and to minimize
variation between ECIS wells. The
electrical stabilization method is very convenient as it is built into the ECIS
software and stabilization is actuated with a simple click of the mouse. That being said, it is necessary to allow 30
minutes or longer after electrical stabilization before the electrodes will
settle into their final equilibration values.
use of cysteine can accomplish the stabilization of the electrodes quickly,
with relatively little effort and does not require the equilibration period
following treatment. To accomplish this, the well should be flooded
with a 10 mM sterile solution of cysteine in water.* The cysteine will form a covalent sulfur-gold
linkage with the electrode surface displacing small molecules that
have adsorbed to gold surface over time and stabilizing the impedance. After exposure (5 minutes or more), the
cysteine solution can be rinsed out of the well, proteins adsorbed if desired,
and the wells inoculated with cells. This cysteine layer provides a hydrophilic substrate that is excellent
for protein adsorption and ultimately cell attachment and spreading.
more information on improving experiment repeatability, please visit the ECIS FAQ page of our website.
from Applied BioPhysics
Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.
Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!