Eagle Biosciences is pleased to introduce the first Intact FGF-21 ELISA assay kit that truly measures the active human intact FGF-21 (1-181) with no cross reaction to any FGF-21 fragments. Other assays that determine fragments of the FGF-21 can overestimate the biological activity of the protein. The physiological functions of FGF-21 require intact molecular structure and amino acid sequence in its N-terminal and C-terminal region.
The Intact FGF-21 ELISA is designed, developed and produced for the quantitative measurement of human intact FGF-21 in serum and EDTA plasma samples. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human intact FGF-21. One antibody specifically binds to the N-terminal human FGF-21 (1-7) and the other specifically binds to the C-terminal human FGF-21 (175-181).
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