 Blood sampling for clinical pathology assessment in mice has always been a challenge, given the limited amount of blood that can be collected both at necropsy and particularly during the live phase of a study. To address this difficulty, and in keeping with our commitment to the 3Rs (replacement, reduction, refinement), Charles River has developed and validated a method permitting a 75% reduction in the total blood volume required for hematology and clinical chemistry assessments in mice. Learn more.
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Introducing Capillary Microsampling
 Many toxicologists and regulators view the mouse as the toxicology model of last resort. Traditional analytical methods and study designs have not allowed for the direct correlation of toxicological effects with drug exposures within the same mouse. Each analysis endpoint (other than pathology) requires blood samples that are a relatively large fraction of the mouse's total blood volume. Consequently, mouse toxicology designs have required the endpoints to be dispersed among large numbers of satellite groups. While covering all standard endpoints required for human drug toxicology, these studies have produced a less-than-satisfactory collage of data.
The advent of new mass spectrometers has meant that instrument sensitivities have increased dramatically, and this, along with the introduction of new endpoints for immunoassay (e.g., MSD), permits the use of the reduced sample volumes without losing assay sensitivity. Learn more.
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| DART Studies in Guinea Pigs
 The scientific and technical staff within our Pennsylvania facility have successfully researched and developed an alternate repeat-dose oral gavage technique that can be utilized in guinea pigs. This technique is now available for use in multiple study designs, including general toxicology assessments and DART studies. Learn more.
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Using Quantitative Whole-Body Autoradiography (QWBA) to Investigate Whole-Body Tissue Distribution in the Minipig
 QWBA is a powerful tool in determining the relative tissue distribution of radiolabelled drugs. This technique, which provides data on the whole-body distribution, can also be used to determine distribution of drugs to specific tissues and localization within target tissues. It is, however, usually restricted to the investigation of distribution in smaller species. In the study described in this poster, the tissue distribution of radioactivity was investigated in the minipig using QWBA techniques. This appears to be the first application of QWBA for this investigation type.
Click here to view our poster on QWBA to learn more.
Click here to listen to our podcast on QWBA.
(Poster and podcast are only available in The SourceSM. Click here to register.)
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mypreclinicalSM
Our new and improved global portal, mypreclinicalSM, offers customizable solutions and increased functionality for secure study tracking. Now available at all of our preclinical facilities. Learn more.
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