As leading experts of BDNF research, the team at Biosensis continue to produce high quality reagents.
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Quantification of (Mature) BDNF
(and mastering the Art and Science of BDNF measurement!)
Dear Doctor,
The Biosensis Team hope you and your colleagues have had a great start to 2016 and wish you a successful year ahead filled with grant approvals, great results and quality publications!
As leading experts of BDNF research products, the team at Biosensis continue to strive to produce high quality products for our customers. We have a great passion for research and are constantly improving our products to allow for the most accurate and reproducible quantification of BDNF possible (we first started talking about this in 2010).
In today's issue of BioSpeak, we focus on a recent comparative study conducted by leading BDNF researchers who demonstrated the superiority of the Biosensis Mature BDNF Rapid™ ELISA kit (BEK-2211-2P) over other BDNF ELISA kits from major suppliers! Please find reference to the paper here.
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Image of our Mature BDNF Rapid™ ELISA kit (BEK-2211-1P). proBDNF interference is minimal and the truncated isoform is not detected!
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Biosensis Mature BDNF Rapid ELISA kit: The Best in Class
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(We always knew it was!)
Low intra/inter-assay, lot-to-lot variability as well as lowest detection limits are important factors of a high-quality ELISA kit. However, a superior ELISA kit combines all of the points above and ties them together with the highest quality standard and a fast assay procedure (i.e. in less than 4 hours!).
Two years after its release (and many happy customers later), the Biosensis Mature BDNF Rapid™ ELISA kit (BEK-2211-2P) has now independently been shown to outperform 5 other commercially available BDNF ELISA kits (Polacchini et. al, 2015). With intra-assay and inter-assay CVs of 1% and 5% respectively, the Biosensis Mature BDNF Rapid™ ELISA kit (BEK-2211-2P) is the most fastest and reproducible sandwich ELISA kit on the market!
Table 1. BDNF ELISA kits performance. Comparison between declared performances and actual tested performances of the specified BDNF ELISA kits. Median and range values, intra- and inter-assay coefficients of variation (CV) were assessed by measuring BDNF serum level from healthy volunteers (n = 38-40). All the kits, except Milliplex, which is based on multiplex technology (Luminex/xMAP), are based on classical sandwich ELISA.
Table 2: Inter-assay variation of the BDNF ELISA kits. Scatter plot showing the BDNF values distribution measured by the same operator on two different days using two plates of the same lot for each brand (Day 1 & Day 2). Each dot represents a BDNF value from one subject and the dashed lines link together two assessments of the same subject. The reproducibility was checked performing one-way ANOVA for repeated measures and the P values are specified.
Biosensis has created a step-by-step 'tutorial' video for all of our Rapid™ ELISA kits on YouTube and we encourage all our ELISA customers to review it here. Our new Mature BDNF Rapid™ ELISA kit is the finest kit on the market for mature BDNF, however proper use and instruction is essential for its success so please check out our ELISA instructional video today.
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Which BDNF Isoform am I Measuring?
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(If you have ever asked yourself this question, keep on reading!)
(If you have NEVER asked yourself this question, continue reading!)
The challenge to quantify the three different BDNF isoforms in blood (mature BDNF, its precursor proBDNF and truncated BDNF) by ELISA is difficult and therefore cannot be quantified using only one assay (Carlino et. al, 2011). The reactivity of proBDNF in the Biosensis Mature BDNF Rapid™ ELISA kit (BEK-2211-2P) is less than 5.7% in weight concentration (or less than 13% in molar concentration - refer to Figure 1), while the truncated BDNF form is not detected by the Mature BDNF Rapid™ ELISA kit. Therefore, the Biosensis Mature BDNF Rapid™ ELISA kit is the optimal choice for quantification of mature BDNF!
Figure 1: Reactivity of proBDNF in the Mature BDNF Rapid™ ELISA kit (BEK-2211-2P) in weight concentration [molar concentration] is Less than 13%!
Figure 2: Reactivity of mature BDNF and proBDNF in the Mature BDNF Rapid™ ELISA kit (BEK-2211-2P). proBDNF shows little to no cross reactivity!
For specific proBDNF quantification, Biosensis offers the proBDNF Rapid™ ELISA kit (BEK-2217-2P), which shows no cross reactivity with either the mature or truncated BDNF forms (Figure 3).
Figure 3: Reactivity of proBDNF and mature BDNF in the proBDNF Rapid™ ELISA kit (BEK-2217-2P). The mature form is not detected!
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With the increase in the literature of mature BDNF as a potential biomarker, the accuracy and reliability for the quantification of BDNF in human samples is essential. Furthermore, there is now a need for consistent and well documented sample preparation procedures. This is reflected by the rapidly growing number of publications and reviews which discuss the association of neurological disorders with changes of BDNF blood levels. For example (Polyakova et. al, 2015), (Carlino et. al, 2013) and (Nurjono et. al, 2012).
Over the coming weeks, Biosensis will release a series of BioSpeak newsletters which will discuss the role of BDNF as a potential biomarker, important aspects related to its quantification in human blood and additional information about BDNF biology. Be on the look out for more BioSpeak newsletters to come!
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If you have a query on any of our products, one of our experienced researchers can help at biospeak@biosensis.com.
Until next time, good luck with your research.
Sincerely,
The Biosensis Team
Contact Information
Biosensis Pty Ltd
Address: 40-46 West Thebarton Road
Thebarton SA 5031 Australia
Email: info@biosensis.comPhone: +61 8 8352 7711 - In the United States, call us in California toll free on 1800 605-5127
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