|
|
|
|
|
|
|
|
|
Biosensis Phospho-Sure™ RTD™ Neuronal Cell & Soft Tissue Extraction Buffer
Dear Doctor,
Welcome to our October edition of Biosensis e-antibody solutions. In this newsletter we are highlighting our popular Phospho-Sure™ Ready-to-Dilute (RTD) Neuronal extraction buffer - optimized for the extraction of phosphorylated proteins from neuronal and other soft tissue types.
P.S. Biosensis is attending the Society for Neuroscience (SFN) conference in Chicago (November 17-21 2015) at Booth #162 and it will be great to see you there!
 Name: Phospho-Sure™ RTD™ Neuronal Cell & Soft tissue extraction buffer .
Description: A new type of extraction buffer which extracts phosphoproteins in a native state without the use of harsh detergents or oxidizers. It is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers (see figure 1). Catalog No: B-100-PSQuantity: 100 mL Form: Powder Price: $87 USD
Figure 1: Shows the decrease in the decay rate in phosphorylated signals from western blots performed on samples held at room temperature for varying times in both Phospho-Sure™ RTD™ (◇) or traditional lysis buffer preparations (●).
Preparation: Preparation of Phospho-Sure™ RTD™ is easy. Simply add sterile ddH2O directly to the powdered solution in the supplied bottle, swirl briefly to dissolve the chemicals and use. The material contains no preservatives so sterile technique should always be used.
Technical Information: Phospho-Sure™ RTD™ is designed as both an isolation and extraction buffer and for best results the intact tissue should be bathed in ice-cold Phospho-Sure™ RTD™ buffer during excision of the tissue. Once the desired tissue piece is isolated in Phospho-Sure™ RTD™ it may be transferred to a small volume of ice-cold Phospho-Sure™ RTD™ for disruption.
Product citations:- Mass E et al, 2014. Murine Creld1 controls cardiac development through activation of calcineurin/NFATc1 signaling. Dev Cell. 31;28(6):711-26.
|
Biosensis offers high quality antibodies, ELISA kits and research reagents to the research community. Please visit our website for a full range of our products at www.biosensis.com.
If you have a query on any of our products, one of our experienced researchers can help at biospeak@biosensis.com.
Until next time, good luck with your research.
Sincerely,
The Biosensis Team
Contact Information Biosensis Pty Ltd
Address: 40-46 West Thebarton Road
Thebarton SA 5031 Australia
Email: info@biosensis.com
Phone: +61 8 8352 7711 - In the United States, call us in California toll free on 1800 605-5127
Web: www.biosensis.com |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
