MLR2 (M-009-100). (see also BEK-2219-2P; BEK-2220-2P) A monoclonal antibody generated more recently by researchers using knockout mice. The immunization protocol was designed to generate antibodies that would be useful across many species such as human, mouse and rat. Despite having only 14 citations, it has strong validation data showing usefulness in many techniques including FACS, Western blot (for native protein only), IHC, immunopanning, sandwich ELISA (when paired with another p75NTR antibody), immunoprecipitation and in vivo targeting for gene delivery.

Flow cytometry analysis of endogenously expressed p75NTR on ShSY5Y cells. X-63 conjugated control IgG represents negative control for all experiments.

Immunofluorescent detection of p75NTR in cultured mouse NSC34 cells using a mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-009-100.

Staining of transverse sections of E9.5 mouse embyros fixed in 4% paraformaldehyde in PBS and immunolabelled with anti-p75 antibody MLR2 (M-009-100, 1:200, green), anti-cleaved-caspase-3 (red, upper panel) and anti-phospho Histone H3 antibodies (PHH3, red, lower panel). Primary antibodies were incubated at 4�C overnight. Scale bar = 200 μm. Courtesy of Dr S. Wiszniak, SA Pathology.

MOAB-2 (M-1586-100). (see also BEK-2215-2P) A new antibody less than 3 years old, so only used in 10 publications to date. However, the researchers who produced this novel Abeta antibody and Biosensis R&D scientists have generated a large amount of validation data for its use in Western blot, IHC and sandwich ELISA for oligomeric Abeta.  

Sample combined cortex and hippocampus from 12 month old 5xFAD mice. 1.25-25 mg of FA extraction were analyzed using the standard Western blot procedure as described in Youmans, Molecular Neurodegeneration, 2012. MOAB-2 was used at a concentration of 1ug/ml.

Immunohistochemical detection of Aβ and APP in coronal sections of the frontal cortex of 1-month old 5xFAD mice. Sections were co-stained with M-1586-100 or APP antibodies (N-terminus and C-terminus). MOAB-2 staining was punctate and did not co-localize with either APP antibodies. Figure courtesy of Youmans et al (2012).