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The Enigmatic p75NTR Neurotrophin Receptor

 

"They seek him here, they seek him there. Those Researchers seek him everywhere.... That damned elusive p75NTR!"

 

(With apologies to Baroness Emmuska Orczy, The Scarlet Pimpernel).  

 

Dear Doctor  

 

Receptors for the neurotrophins were identified almost 50 years ago using simple biochemical techniques such as the use of radioactive NGF to find specific binding proteins. p75NTR was the first neurotrophin receptor to be cloned, followed some time later by the identification of the Trk family of receptor kinases as high affinity neurotrophin receptors. The activation of these kinase receptors by all members of the neurotrophin family led to clarity in assigning signaling pathways from the Trks to biological functions particularly neuronal survival.

 

In contrast, p75NTR signaling has yielded its secrets very slowly. Evidence for p75NTR activation of the NF-kB pathway eventually was presented by Carter et al. (1996). This was followed after long intervals by identification of cleavages to the extracellular and intracellular domains of the receptors, death domains and co-receptors. The demonstration of a biological action of the pro-neurotrophins has led to a new wave of activity as evidence builds for a pro-NT/ p75NTR / sortilin complex that can trigger cell death. An excellent recent review highlights the role of this complex in the regulation of pain both with, and independently of, the TrkA receptor Lewin and Nykjaer (2014).

 

Another great review on p75NTR signaling and resultant biological functions can be found the most recent issue of the Handbook of Experimental Pharmacology, which is entirely devoted to Neurotrophic Factors Kraemer, Yoon and Carter (2014).

  

Hot topics in p75NTR research
The role of p75NTR in pain signaling
p75NTR as a biomarker
p75NTR and beta Amyloid peptide
Biosensis p75NTR and related products
The Biosensis Unconditional Guarantee
New NGFR/p75ECD Rapid™ ELISA Kit
hu p75 standard curve
Standard curve of our new
NGFR/p75ECD Rapid™ ELISA Kit: (Catalog No# BEK-2219-2P). 
 

The role of p75NTR in pain signaling 

 

While NGF stimulation of TrkA is well known to trigger pain in sensory neurons (and antibodies to NGF are a potent treatment for many types of pain), Lewin and Nykjaer (2014) have critically reviewed the evidence for a pro-NT / p75NTR / sortilin complex, and proposed a possible role in both pain and sensory neuropathies. 

 

p75NTR as a biomarker

 

The first indication that p75NTR could function as a useful biomarker of disease or trauma was published 26 years ago DiStefano and Johnson (1988)A recent publication has progressed these early observations by showing a very strong correlation between p75NTR and disease progression in ALS (Amyotrophic Lateral Sclerosis) patients Shepheard et al (2014)This bodes well for both early diagnosis and drug development. 

 

p75NTR and beta Amyloid peptide


The intriguing finding that the neutotoxic effects of amyloid beta peptide are at least partially mediated by p75NTR is now understood to occur via a complex of the receptor with the Death Receptor-6 (DR6) protein Hu et al. (2013)This finding provides yet another option for drug development in Alzheimer's disease, although the story is complicated by the growth-promoting action of the beta-amyloid precursor protein on p75NTR Hasebe et al. (2013).

 

These findings further highlight that it will take many more years of intensive study before the mysterious p75NTR yields all of its secrets.....

 

Biosensis p75NTR and related products

 

Biosensis is excited to advise the release of the latest member of our Rapid™ ELISA Kits for the detection and quantification of p75NTR. This new kit complements the highly popular Neurotrophin and ProNeurotrophin kits in the Rapid™ range.

 

NGFR/p75ECD Rapid™ ELISA Kit: Human 

 

Image: Standard curve of our new NGFR/p75ECD Rapid™ ELISA Kit: Human (Catalog No# BEK-2219-2P).

 

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay. 

 

 

Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a

MLR2  

Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a] (Catalog No# M-009-100). Image: Immunofluorescent staining of adult rat mesenteric nerves following in vivo intraperitoneal administration of labeled MLR2. This popular monoclonal is also available with a FITC label (Catalog No# M-018-100-FT). 

 

 

 

 

Mouse monoclonal antibody to rat p75NTR [MC192]: IgG 

M-006-100  

Image: Immunofluorescent detection of p75NTR [MC192] in cultured rat dorsal root ganglion (DRG) using Mouse monoclonal antibody to rat p75NTR [MC192] (Catalog No# M-006-100). This popular monoclonal is also available with a FITC label (Catalog No# M-016-100-FT).

 

 

 

 

 

Rabbit antibody to Sortilin (neurotensin receptor 3): whole serum

R-150-100  

Image: IHC on rat DRG (free floating cryo section) using Rabbit antibody to Sortilin (neurotensin receptor 3): whole serum (Catalog No# R-150-100) at a dilution of 1: 1000. 

 

 

 

 

 

 

The Biosensis UNCONDITIONAL Guarantee
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Should your manuscript ever be rejected by ANY journal because of insufficient information about a Biosensis antibody, we will gladly refund TWICE the purchase price of that antibody!


By researchers for researchers!   

The staff at Biosensis have over 60 years of combined experience in manufacturing and working with antibodies and have published over 340 peer reviewed research publications in the field of neuroscience. We guarantee to provide you with the antibody information you need to get your research published.

 

If you have a query on any of our products, one of our experienced researchers can help at biospeak@biosensis.com.
   
Until next time, good luck with your research.
 
Sincerely,
Tina
 
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