Biosensis Phospho-Sure

Biosensis Phospho-Sure™ RTD™ Neuronal Cell & Soft Tissue Extraction Buffer

 

Dear Doctor       

Welcome to our April edition of Biosensis e-antibody solutions. April has been a exciting month for Biosensis as we have been busy preparing the launch of our new range of products. We are pleased to introduce you to our newly released Phospho-Sure™ Ready-to-Dilute (RTD™) Neuronal extraction buffer - optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types.

 

Biosensis' new Phospho-Sure™ Ready-to-Dilute (RTD™) Neuronal extraction buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers (see figure 1).

 

Phospho-Sure™ RTD™ is designed as both an isolation and extraction buffer and for best results the intact tissue should be bathed in ice-cold Phospho-Sure™ RTD™ buffer during excision of the tissue. Once the desired tissue piece is isolated in Phospho-Sure™ RTD™ it may be transferred to a small volume of ice-cold Phospho-Sure™ RTD™ for disruption.

 

Biosensis Phospho-Sure  

Figure 1: Shows the decrease in the decay rate in phosphorylated signals from western blots performed on samples held at room temperature for varying times in both Phospho-Sure™ RTD™ or traditional lysis buffer preparations.  

 

Figure 2: Western blots of CREB-P, SAP/JNK-P, ERK1/2-P and ERK1/2 from ICc. Samples of the adult guinea pig central nucleus of the inferior colliculus (ICc) were homogenized in Phospho-Sure™ RTD™ or traditional Tris-HCL triton X-100 lysis buffer and kept on ice for up to 120 min. Aliquots of the homogenates, each containing 30μg protein, were run on SDS-PAGE. Separated proteins were electrophoretically transferred to the blotting membrane, which was incubated with anti-CREB-P antibody. The blot was stripped and treated with anti-SAP/JNK-P, ERK1/2-P or ERK1/2 antibodies.

Photo courtesy of Suneja et al., Journal of Neuroscience Methods 150 (2006) 238-241).


Preparation of Phospho-Sure™ RTD™ is very simple. Simply add sterile ddH2O directly to the powdered solution in the supplied bottle, swirl briefly to dissolve the chemicals and use. The material contains no preservatives so sterile technique should always be used.

 

Product Description                       Catalog Number                    Quantity

Phospho-Sure™ RTD™                   B-100-PS                                100 mL

Phospho-Sure™ RTD™                   B-200-PS                                200 mL


 When you want the best reagents, trust Biosensis 

 

The staff at Biosensis have many years of combined experience in manufacturing and working with antibodies and have published over 340 peer reviewed research publications in the field of neuroscience. We guarantee to provide you with the antibody information you need to get your research published.
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