Biosensis Phospho-Sure™ RTD™ Neuronal Cell & Soft Tissue Extraction Buffer
Dear Doctor
Welcome to our April edition of Biosensis e-antibody solutions. April has been a exciting month for Biosensis as we have been busy preparing the launch of our new range of products. We are pleased to introduce you to our newly released Phospho-Sure™ Ready-to-Dilute (RTD™) Neuronal extraction buffer - optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types.
Biosensis' new Phospho-Sure™ Ready-to-Dilute (RTD™) Neuronal extraction buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers (see figure 1).
Phospho-Sure™ RTD™ is designed as both an isolation and extraction buffer and for best results the intact tissue should be bathed in ice-cold Phospho-Sure™ RTD™ buffer during excision of the tissue. Once the desired tissue piece is isolated in Phospho-Sure™ RTD™ it may be transferred to a small volume of ice-cold Phospho-Sure™ RTD™ for disruption.
Figure 1: Shows the decrease in the decay rate in phosphorylated signals from western blots performed on samples held at room temperature for varying times in both Phospho-Sure™ RTD™ ♢ or traditional lysis buffer preparations ●.
Figure 2: Western blots of CREB-P, SAP/JNK-P, ERK1/2-P and ERK1/2 from ICc. Samples of the adult guinea pig central nucleus of the inferior colliculus (ICc) were homogenized in Phospho-Sure™ RTD™ or traditional Tris-HCL triton X-100 lysis buffer and kept on ice for up to 120 min. Aliquots of the homogenates, each containing 30μg protein, were run on SDS-PAGE. Separated proteins were electrophoretically transferred to the blotting membrane, which was incubated with anti-CREB-P antibody. The blot was stripped and treated with anti-SAP/JNK-P, ERK1/2-P or ERK1/2 antibodies.
Photo courtesy of Suneja et al., Journal of Neuroscience Methods 150 (2006) 238-241).Preparation of Phospho-Sure™ RTD™ is very simple. Simply add sterile ddH2O directly to the powdered solution in the supplied bottle, swirl briefly to dissolve the chemicals and use. The material contains no preservatives so sterile technique should always be used. Product Description Catalog Number Quantity Phospho-Sure™ RTD™ B-100-PS 100 mL Phospho-Sure™ RTD™ B-200-PS 200 mL
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