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Dear Doctor       

We would like to welcome you to our special Holiday edition of Biosensis e-antibody solutions and introduce you to our newly released Amylo-Glo® RTD™ 'Ready-to-Dilute' Staining Kits. Light up your Holidays with Biosensis Amylo-Glo® Staining Kits - a novel fluorescent histochemical marker for localization of amyloid plaques in brain tissue.   

 

Amylo_Glo  

  • Image: This triple exposure allows for the simultaneous localization of Amylo-Glo positive amyloid plaques (blue), GFAP positive hypertrophied astrocytes (green) and activated microglia (red) in the hippocampus of the AD/Tg mouse. Combined UV, blue and green light illumination.     

Biosensis is pleased to offer our new Amylo-Glo® RTD™ 'Ready-to-Dilute' Staining reagent - designed to stain amyloid plaques in tissue sections (Catalog No: TR-300-AG). This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120-126). 


Amylo-Glo® will not bleed through when illuminated with other filters
Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescentlabeling studies. 

Amylo_Glo images
  • Image 1: High magnification view of Amylo-Glo® labeled amyloid plaque from the cortex of an 11 month old AD/Tg mouse with UV il- lumination only. Note the high clarity, ultra low background and very fine detail available only with Biosensis Amylo-Glo® RTD staining reagent.
  • Image 2: Congo Red stain of amyloid plaque with green light excitation. The central region of the plaque appeared uniformly bright and fibrils but fine details remain obscure. The intensity of the fluorescent emission is the weakest of all tracers though background is also low. 
  • Image 3: Thioflavin S staining of amyloid plaque with blue light excitation The central region of the plaque is uniformly stained but with diffuse appearing fibrils. The background staining is relatively high, with some relatively faint cellular labeling as well, leading to more difficult plaque quantification and clarity determination.  

Compatible with fresh, frozen, and formalin-fixed immunohistochemistry
   
Amylo-Glo® is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily.

Superior visualization of amyloid plaques than other commonly used stains  
Amylo-Glo® RTD™ is a brighter, longer lasting, higher definition plaque staining platform that will make quantitative plaque determination better, easier, and faster.   
Also available: New Ethidium Bromide RTD™ Staining Kit

Also available is the new Amylo-Glo® - Ethidium Bromide (EB) RTD™ Staining Kit. The EB option allows visualization of both cell bodies/nuclei (red) and plaques under UV illumination for easy visual mapping (Catalog No: TR-400-AG).

 

Amylo_Glo4  

  • Image: Combined Amylo-Glo® labeling of amyloid plaques (blue) with ethidium bromide Nissl (cell body) counterstain (red) in the dentate gyrus region of the hippocampus of the AD/Tg mouse. Combined UV and Green light excitation.

Biosensis is pleased to bring you these new and exciting 'Ready-to-Dilute' Staining Kits to the market and we encourage all that have an interest in localizing amyloid plaques to try it.

 

 

Amylo-Glo® is a registered trademark of Histo-Chem, Inc.  

Photos courtesy of Dr. Larry Schmued and reprinted from Journal of Neuroscience Methods 209 (2012) 120-126.        


 When you want the best reagents, trust Biosensis 

 

The staff at Biosensis have many years of combined experience in manufacturing and working with antibodies and have published over 340 peer reviewed research publications in the field of neuroscience. We guarantee to provide you with the antibody information you need to get your research published.
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If you have a query on any of our products, please contact us at biospeak@biosensis.com.
 
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