Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescentlabeling studies.
- Image 1: High magnification view of Amylo-Glo® labeled amyloid plaque from the cortex of an 11 month old AD/Tg mouse with UV il- lumination only. Note the high clarity, ultra low background and very fine detail available only with Biosensis Amylo-Glo® RTD staining reagent.
- Image 2: Congo Red stain of amyloid plaque with green light excitation. The central region of the plaque appeared uniformly bright and fibrils but fine details remain obscure. The intensity of the fluorescent emission is the weakest of all tracers though background is also low.
- Image 3: Thioflavin S staining of amyloid plaque with blue light excitation The central region of the plaque is uniformly stained but with diffuse appearing fibrils. The background staining is relatively high, with some relatively faint cellular labeling as well, leading to more difficult plaque quantification and clarity determination.
Compatible with fresh, frozen, and formalin-fixed immunohistochemistry
Amylo-Glo
® is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo
® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily.
Superior visualization of amyloid plaques than other commonly used stains
Amylo-Glo® RTD™ is a brighter, longer lasting, higher definition plaque staining platform that will make quantitative plaque determination better, easier, and faster.
