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Dear Doctor
Welcome to our November addition of Biosensis e-antibody solutions. This newsletter highlights the advantages of using chicken primary antibodies and our range of neuronal cell markers. To view our whole catalog please visit us at www.biosensis.com.
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So why use Chicken antibodies?
Chicken host antibodies offer a number of advantages:
- Chicken antibodies are harvested from egg yolk, thus avoiding the need to collect blood from an animal. This is a more humane way to make antibodies.
- Chicken proteins are phylogenetically distinct from mammals which allows for the development of antibodies to highly conserved mammalian antigens.
- Chicken antibodies provide researchers with the ability to perform double or triple labeling experiments with rabbit and mouse host antibodies.
- Purified chicken IgY is extremely stable and can be stored for long periods of time at 2-8°C.
- The use of chicken antibodies can help to minimize background issues due to the fact that they do not react with mammalian Fc-receptors.
Our range of chicken neuronal cell markers include the following:
Chicken polyclonal antibody to Microtubule Associated Protein MAP2
The image is a thin section of adult rat cerebellum, showing the molecular layer (outside of lobe), granular layer (blue since it's full of small neurons) and the white matter in the middle. MAP2 staining of neuronal dendrites is green (chicken anti-MAP2, C-1382-50) GFAP staining is red (rabbit anti-GFAP, R-1374-50) and blue staining is DNA.
Also available - Mouse monoclonal antibody to Microtubule Associated Protein 2 (MAP2) (M-1625-100).
Chicken polyclonal antibody to Vimentin
 View of neonatal rat brain cultures stained with chicken anti-Vimentin (red, C-1409-50) and rabbit GFAP (green, R-1374-50). Since these two proteins are found only in non-neuronal cells so you don't see any neurons, except for their nuclei in blue. Maturish astrocytes have only GFAP so appear green or have a mix of both proteins, so appear yellow. Some cells only have the vimentin (immature astrocytes, microglia and fibroblasts) and so appear red.
Chicken polyclonal antibody to Neurofilament Light (NF-L)
Mixed neuron/glial cultures stained with Chicken polyclonal antibody to NF-L C-1390-50 (red) and Rabbit polyclonal antibody to Neurofilament Heavy, phosphorylated R-1388-50 (green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated Neurofilament Heavy (NF-H) has a much rmore restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red. Also available - Mouse anti-NF-L (M-1391-50) and Rabbit anti-NF-L (R-1392-50).
Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)
 Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
Also available - Mouse anti-GFAP (M-1375-100) and Rabbit anti-GFAP (R-1374-50).
Chicken polyclonal antibody to Neurofilament Medium (NF-M)
 View of mixed neuron/glial cultures stained with chicken polyclonal antibody to NF-M C-1393-50 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
Also available - Mouse anti-NF-M (M-1394-100) and Rabbit anti-NF-M (R-1395-50).
To view the whole range of our chicken antibodies - including chicken anti-BDNF (C-1517-500) please click here.
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When you want the best reagents, trust Biosensis
The staff at Biosensis have over 60 years of combined experience in manufacturing and working with antibodies and have over 340 peer reviewed research publications in the field of neuroscience. We guarantee to provide you with the antibody information you need to get your research published.
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