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| The ECIS Connection - March 2014 |
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May 29 - 30, 2014
Applied BioPhysics is offering a two day in depth ECIS training course. The course will include both lecture and wet lab training. The training will take place at the Applied BioPhysics facility in Troy, NY. Training will be conducted by Dr. Charles Keese, Dr. Ivar Giaever, Dr. Christian Renken, Dr. Judith Stolwijk and Catherine Toniatti-Yanulavich.
Attendance will be limited to a maximum of 6 people.
Cost is $1,250 per person. This includes two nights of lodging, transportation to and from the hotel to the Applied BioPhysics facility, lunch on both days and dinner Thursday evening. To register, contact Wendy Ladouceur for a registration form at 518-880-6860, ladouceur@biophysics.com or download the form from our website, biophysics.com.
ECIS-related topics to be covered include:
- Experimental design and array selection
- Array preparation and stabilization
- Obtaining good well-to-well and experiment-to-experiment repeatability
- Applying extracellular matrix proteins to the electrodes
- Techniques for array inoculation
- Techniques for addition of compounds to ECIS wells
- Basics of the ECIS software for data acquisition
- Advanced features of the ECIS software for data acquisition
- Basics of the data analysis software
- Advanced features of the data analysis software
- Basics of impedance measurements
- Theory behind ECIS
- Simple and complex impedance and the value of R and C in cell measurements
- Selecting the AC frequency or frequencies for experiments
- Modeling ECIS data
- In situ electroporation
- Cell migration measurements with the ECIS "wound-healing" assay
- Cell migration measurements with the "electric fence"
- A survey of cell biology applications using ECIS
- Fluorescence staining of cells on the ECIS array
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ECIS Software
Latest Software version:
The latest version of the software is v1.2.50 available from:
http://www.biophysics.com/software/ECIS_Software_v1_2_150.msi
Software Tips:
1) When exporting a graph for use in a presentation or paper you can change the font size in the software. First click the Export button to get a separate window. Then go to File | Export Setup, click Fonts and you can specify the font name, size and properties. Then click Apply to Figure. You can now save the graph to an image file, or copy to the clipboard.
2) If you wish to keep the axis range fixed between plots you can set the X and Y range under Display Options in the bottom right of the GUI. Now with the latest software, you can also fix the Z range when displaying 3D plots of MFT datasets.
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ECIS® trans-Filter Adapter Application Note
For ECIS® Z Theta instruments
using software version 1_2_150 or higher
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ECIS p-Flow Peristaltic Pump
The Model ECIS p-Flow can be controlled manually to adjust flow rate from -20ml/min to +20ml/min with a minimum flow rate of 70ul/min. Direction of the pump can be adjusted via a toggle switch or via the ECIS software (V 1.2.151 or higher).
The pump can also be controlled automatically from within the ECIS software (V 1.2.150 or higher) via a USB or RS232 connection. The pump can be programmed to run continuous or ramp up and down. It can store complex programmed flow profiles and run them disconnected from the PC. Each pump has a unique serial number so multiple pumps can be run from one PC by using a USB hub if required.
Performance specifications:
Maximum Flow Rate with 3mm ID tubing: 20ml/min
Minimum Flow Rate with 3mm ID tubing: 70ul/min (1.2uL/sec)
Flow Rate resolution with 3mm ID tubing: 70ul/min from -20ml/min to +20ml/min
Max ramp rate 0-20ml/min: 150msec (forward or reverse)
Full forward to full reverse, -20ml/min to +20ml/min: 400msec
Pump Speed: -60 to +60 RPM stepper driven motor
Pump Resolution: 1024 increments/rev
Control Input: USB or RS232
Dimensions: 4.12"W x 4.12"H x 6.25"L
Case: Powder Coated Aluminum
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Early Career Mini-Grants
Applied BioPhysics would like to help young scientists obtain funding. The ECIS mini-grant is aimed at early career scientists who are applying for their first RO1 grant. For a researcher wanting to use ECIS technology to achieve their research goals, Applied BioPhysics will provide an ECIS instrument, ECIS arrays, and consultation in order to generate preliminary data to support the applicants RO1 proposal. Interested scientists should submit their research plan with a cover letter explaining how ECIS technology can be used to achieve their specific objectives. Applied BioPhysics will evaluate proposals based on scientific merit, suitability with ECIS technology and novelty.
To apply please send a resume, RO1 research plan and cover letter to Christian Renken at renken@biophysics.com. |
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ECIS Webinar Schedule 2014
ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.
All webinars are held at 11:00am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com and scroll to the webinar date of interest. Real-time Electroporation and Monitoring - 11:00 AM EST
March 11, 2014
Cell Attachment and Spreading Measurements - 11:00 AM EST
March 25, 2014
Signal Transduction Assays - 11:00 AM EST
April 8, 2014
Toxicology with ECIS - 11:00 AM EST
April 22, 2014
ECIS Theory - 11:00 AM EST
May 6, 2014
Cell Invasion / Extravasation Assays - 11:00 AM EST
May 20, 2014
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New Publications
LMX1B is Essential for the Maintenance of Differentiated Podocytes in Adult Kidneys Tillmann Burghardt, Jürgen Kastner, Hani Suleiman, Eric Rivera-Milla, Natalya Stepanova, Claudio Lottaz, Marion Kubitza, Carsten A. Böger, Sarah Schmidt, Mathias Gorski, Uwe de Vries, Helga Schmidt, Irmgard Hertting, Jeffrey Kopp, Anne Rascle, Markus Moser, Iris M. Heid, Richard Warth, Rainer Spang, Joachim Wegener, Claudia T. Mierke, Christoph Englert, and Ralph Witzgall J. Am. Soc. Nephrol. 2013; 24:1830-1848.
Ca2+ and calpain control membrane expansion during the rapid cell spreading of neutrophils Sharon Dewitt, Robert J. Francis, and Maurice B. Hallett J. Cell Sci. 2013; 126:4627-4635.
Loss of Phosphatase and Tensin Homolog (PTEN) Induces Leptin-mediated Leptin Gene Expression: FEED-FORWARD LOOP OPERATING IN THE LUNG Ravi Ramesh Pathak, Aditya Grover, Prerna Malaney, Waise Quarni, Ashish Pandit, Diane Allen-Gipson, and Vrushank Davé J. Biol. Chem. 2013; 288:29821-29835.
Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization Koji Ando, Shigetomo Fukuhara, Takahiro Moriya, Yutaro Obara, Norimichi Nakahata, and Naoki Mochizuki J. Cell Biol. 2013; 202:901-916.
Src Family Kinases as Novel Therapeutic Targets to Treat Breast Cancer Brain Metastases Siyuan Zhang, Wen-Chien Huang, Lin Zhang, Chenyu Zhang, Frank J. Lowery, Zhaoxi Ding, Hua Guo, Hai Wang, Suyun Huang, Aysegul A. Sahin, Kenneth D. Aldape, Patricia S. Steeg, and Dihua Yu Cancer Res. 2013; 73:5764-5774.
Triptolide-induced Cell Death in Pancreatic Cancer Is Mediated by O-GlcNAc Modification of Transcription Factor Sp1 Sulagna Banerjee, Veena Sangwan, Olivia McGinn, Rohit Chugh, Vikas Dudeja, Selwyn M. Vickers, and Ashok K. Saluja J. Biol. Chem. 2013; 288:33927-33938.
Enhancement Of Endothelial Cell Barrier Function By Antibody-Driven Affinity Modulation Of PECAM-1
Heng Mei, Cathy Paddock, Jay Campbell, Ralph Albrecht, and Peter J. Newman Blood. 2013; 122:193.
Pivotal Role Of Heat Shock Proteins In Monocyte and Endothelial Cell Pathology In Sickle Cell Disease
Nagavedi S. Umapathy, Kavita Natrajan, Abdullah Kutlar, Steffen E. Meiler, and Julia E. Brittain Blood. 2013; 122:969.
Oxidant-induced corticosteroid unresponsiveness in human bronchial epithelial cells Irene Heijink, Antoon van Oosterhout, Nathalie Kliphuis, Marnix Jonker, Roland Hoffmann, Eef Telenga, Karin Klooster, Dirk-Jan Slebos, Nick ten Hacken, Dirkje Postma, and Maarten van den Berge Thorax. 2014; 69:5-13.
eNOS-derived nitric oxide regulates endothelial barrier function through VE-cadherin and Rho GTPases
Annarita Di Lorenzo, Michelle I. Lin, Takahisa Murata, Shira Landskroner-Eiger, Michael Schleicher, Milankumar Kothiya, Yasuko Iwakiri, Jun Yu, Paul L. Huang, and William C. Sessa J. Cell Sci. 2013; 126:5541-5552.
Limited Role of Nuclear Receptor Nur77 in Escherichia coli-Induced Peritonitis Anouk A. J. Hamers, Sven Uleman, Claudia M. van Tiel, Daniëlle Kruijswijk, Anne-Marieke van Stalborch, Stephan Huveneers, Carlie J. M. de Vries, and Cornelis van 't Veer Infect. Immun. 2014; 82:253-264.
PDLIM2 regulates transcription factor activity in epithelial-to-mesenchymal transition via the COP9 signalosome Rachael A. Bowe, Orla T. Cox, Verónica Ayllón, Emilie Tresse, Nollaig C. Healy, Shelley J. Edmunds, Merei Huigsloot, and Rosemary O'Connor Mol. Biol. Cell. 2014; 25:184-195.
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Have you recently published an article that includes the use of ECIS? If so, submit your publications to Applied BioPhysics via email to Dr. Christian Renken at renken@biophysics.com. We will announce your article in our newsletter, post it on our website and send you two FREE 8 well PET arrays!
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Upcoming Events
Representatives from Applied BioPhysics will be at the following tradeshows and events:
5th International Meeting on Angiogenesis
March 12 - 14, 2014
Amsterdam, The Netherlands
with ibidi GmbH
The 29th Joint Annual Conference of Biomedical Science
March 15 - 16, 2014
Taipei, Taiwan
with Sunpoint Scientific Instrument Co., Ltd.
Society of Toxicology
March 23 - 27, 2014
Phoenix, AZ
7th Annual Conference: Biointerfaces in Biomaterials
March 24 - 28, 2014
Aarhus, Denmark
with ibidi GmbH
Analytica 2014
April 1 - 4, 2014
Munchen, Germany
with ibidi GmbH
American Association for Cancer Research
April 5 - 9, 2014
San Diego, CA
The 18th International Vascular Biology Meeting (IVBM 2014)
April 14 - 17, 2014
Miyakomesse (Kyoto)
with Nepa Gene Co., Ltd.
Experimental Biology
April 26 - 30, 2014
San Diego, CA
Korea Society for Biochemistry and Molecular Biology (KSBMB)
May 14th - 16th, 2014
Seoul, South Korea
with LeeBaeg Scientific Co.,Ltd
ATS 2014 International Conference
May 16 - 21, 2014
San Diego, CA
14th International ELMI Meeting
May 20 - 23, 2014
Oslo, Norway
with ibidi GmbH
Annual Meeting of Japan Society for Bioscience,
Biotechnology, and Agrochemistry 2014
May 28 - 30, 2014
Meiji University, Ikuta Campus
with Nepa Gene Co., Ltd.
FASEB
Calcium & Cell Function
June 1 - 6, 2014
Nassau, Bahamas
Gorden Research Conference
Barriers of the CNS
June 15 - 20, 2014
New London, NH
3D Cell Culture 2014
Advanced Model Systems, Applications & Enabling Technologies
June 25 - 27, 2014
Freiburg, Germany
with ibidi GmbH
Gorden Research Conference
Signal Transduction by Engineered Extracellular Matrices
July 6 - 11, 2014
Waltham, MA
Gorden Research Conference
Endothelial Cell Phenotypes in Health & Disease
July 6 - 11, 2014
Girona-Costa Brava, Spain
FASEB
The Lung Epithelium in Health and Disease
July 27 - August 1, 2014
Saxtons River, VT
11th ISRA: From Molecular Machinery to Clinical Challenges
September 7 - 11, 2014
Banff, Canada
Korea Society for Molecular and Cellular Biology (KSMCB)
Oct 9th - 11th, 2014
Seoul, South Korea
with LeeBaeg Scientific Co.,Ltd
The 87th Annual Meeting of the Japanese Biochemical Society
October 15 - 18, 2014
Kyoto International Conference Center
with Nepa Gene Co., Ltd.
NAVBO
Vascular Biology 2014
Oct 19 - 23, 2014
Monterey, CA
American Society for Cell Biology
December 6 - 10, 2014
Philadelphia, PA
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Tip of the Month: Condensation Issues with the ECIS Station
With the introduction of multiplexer switching in the Z and ZTheta ECIS Models, a portion of the ECIS electronics had to be moved into the incubator space. Theses electronics are specially treated with a waterproof conformal coating to deal with the high humidity required for tissue culture. Nevertheless, there are situations where water can condense directly upon the electronics as well as upon the pogo pins used to contact the arrays. This occurs when a room temperature Station is moved into the 37C humid incubator resulting in condensation of water vapor upon the relatively cool Station. This liquid water will ultimately evaporate from the surfaces and no harm is done. While the condensate is present, there may be some temporary electrical shorting of the pogo pins as well as some other metallic connections that must remain exposed. When this happens, one may see lower than expected values in impedance and resistance and higher capacitance values. As soon as the surfaces dry in the incubator, these readings will return to normal.
Clearly it is best to not have condensates form on Station, and in spite of effort to protect the circuits, obviously electronics and water don't go well together. Condensation can easily be avoided. First, by always storing the Station in the warm incubator it will remain dry. For many laboratories this is not a practical solution and it is necessary to move the Station in and out of the incubator space. In this case, if one places the Station and its leads in a plastic bag while it warms to 37C, it can then be removed from the bag and connected to the Station Controller. With this care, water will not condense upon the already warmed surfaces. |
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ECIS Humor
Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.
Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!
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