The ECIS Connection - July 2013              


ECIS Training Schedule


October 3 & 4, 2013


Applied BioPhysics is offering a two day in depth ECIS training course. The course will include both lecture and wet lab training. The training will take place at the Applied BioPhysics facility in Troy, NY. Training will be conducted by Dr. Charles Keese, Dr. Ivar Giaever, Dr. Christian Renken, Dr. Judith Stolwijk and Catherine Toniatti-Yanulavich.


Attendance will be limited to a maximum of 6 people.


Cost is $1,250 per person. This includes two nights of lodging, transportation to and from the hotel to the Applied BioPhysics facility, lunch on both days and dinner Thursday evening. To register, contact Wendy Ladouceur for a registration form at 518-880-6860, ladouceur@biophysics.com or download the form from our website, biophysics.com.


ECIS-related topics to be covered include: 

  • Experimental design and array selection
  • Array preparation and stabilization
  • Obtaining good well-to-well and experiment-to-experiment repeatability
  • Applying extracellular matrix proteins to the electrodes
  • Techniques for array inoculation
  • Techniques for addition of compounds to ECIS wells
  • Basics of the ECIS software for data acquisition
  • Advanced features of the ECIS software for data acquisition
  • Basics of the data analysis software
  • Advanced features of the data analysis software
  • Basics of impedance measurements
  • Theory behind ECIS
  • Simple and complex impedance and the value of R and C in cell measurements
  • Selecting the AC frequency or frequencies for experiments
  • Modeling ECIS data
  • In situ electroporation
  • Cell migration measurements with the ECIS "wound-healing" assay
  • Cell migration measurements with the "electric fence"
  • A survey of cell biology applications using ECIS
  • Fluorescence staining of cells on the ECIS array 


Now available from Springer Publishing  


Electric Cell-Substrate Impedance Sensing and Cancer Metastasis

Jiang, Wen G. (Ed.) 

2012, VIII, 260 p. 76 illus., 60 in color. 

Available Formats: e-Book $119.20 & Hardcover $151.20 

 ECIS Book

About this book


The first book devoted to cell based impedance sensing

  • Written by experts in this area
  • Highly informative colour figures
  • Protocols and methods

    Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.

    Content Level » Research

    Keywords »Cancer - Cell biology - Cell signalling - Impedance Sensing - Metastasis

    Related subjects »Biomedical Sciences - Cancer Research - Molecular Medicine - Oncology & Hematology


    Table of Contents



    1. Electric Cell-substrate Impedance Sensing Concept to Commercialisation. 

    2. Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell    Impedance. 

    3. ECIS as a tool in the study of metastasis suppressor genes: Epithelial Protein Lost In Neoplasm   (EPLIN).

    4. Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening.

    5. Epithelial-mesenchymal transition and the use of ECIS.

    6. Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing.

    7. Tight Junctions in Cancer Metastasis and their investigation using ECIS (Electric Cell-substrate Impedance Sensing).

    8. Epithelial wound healing and the effects of cytokines investigated by ECIS.

    9. Tumour-endothelial and tumour-mesothelial interactions investigated by impedance sensing based cell analyses.

    10. Application of Electric Cell-Substrate Impedance Sensing in evaluation of traditional medicine on the cellular functions of gastric and colorecctal cancer cells.

    11. Electric cell-substrate impedance sensing as a screening tool for wound healing agents.

    12. ECIS, cellular adhesion and migration in keratinocytes. 13. Current and future application of ECIS models to study bone metastasis.







    Conference Deadlines 

    July 1

    Abstract submission to be considered for oral presentation

    Aug. 1

    Abstract submission to be included in conference proceedings

    Aug. 1

    Registration for pre and post meeting events


    Biological Sessions

    Barrier Function

    Signal Transduction

    Cell Proliferation

    Cell Migration

    Cell Spreading




    Technical Sessions

    Advanced Data Analysis

    New Electrode Designs

    ECIS - X Techniques 



    Wednesday August 21, 2013 at 12:30 PM CEST
    Friday August 23, 2013 at 12:30 PM CEST
    Add to Calendar




    Semmelweis University, Budapest, Hungary
    Nagyvarad ter 4.
    Budapest H-1089


    Driving Directions


    On behalf of the Scientific Committee and myself I would like to invite you to the 2nd International Conference on Impedance Based Cellular Assays in Budapest, Hungary on August 21 - 23.


    Nearly two years have passed since the first IBCA meeting that brought together people using electrical impedance measurements to study cells in vitro. Our aim is to link together the growing number of researchers in this area, to share ideas and protocols and to discuss future directions of the technology.


    As the host of IBCA2013 I would like to invite you and your colleagues who have an interest in non-invasive cell monitoring to join us and to be part of this year's meeting. Please save the date of August 21st to 23rd.


    The beautiful city of Budapest will hopefully set the stage for another round of inspiring presentations and fruitful discussions about this most versatile way of whole-cell biosensing.


    I look forward to welcome you all in Budapest for IBCA2013


    Laszlo Kohidai  


    Register Now!

    I can't make it


    Confirmed Speakers


    Nikolaj Gadegaard


    Dennis Grab


    Klaus Groschner


    Sven Ingebrandt


    Kiyoshi Itagaki


    Andreas Janshoff


    Wen Jiang


    Laszlo Kohidai


    Orsolya Lang


    Hamed Laroui


    Chun-Min Lo


    Stefanie Michaelis


    Geerten v. Nieuw Amerongen


    Phillipe Renaud


    Martina Schmidt


    Israel Sekler


    Mohamed Trebak


    Joachim Wegener






    ECIS Software   


    Latest Software version:

    The latest version of the ECIS software is v1.2.126 and available for download from:




    Software Tip


    During an experiment it is normally best to just observe progress as it is displayed on the screen to ensure system stability. By default the toolbar is locked when an experiment is started to prevent unwanted operations. If necessary, the toolbar can be unlocked by clicking the 'Lock' toolbar icon. It is recommended that the toolbar is then re-locked.


    If you try to open the current experiment file during data collection then you may find difficulties when reopening the file later. The experimental data may appear to have stopped prematurely. In most cases it is possible to recover the file. Please contact info@biophysics.com if you have this problem and for information about what to do.

     Early Career Mini-Grants


    Mini Grant Array Image  



    Applied BioPhysics would like to help young scientists obtain funding. The ECIS mini-grant is aimed at early career scientists who are applying for their first RO1 grant. For a researcher wanting to use ECIS technology to achieve their research goals, Applied BioPhysics will provide an ECIS instrument, ECIS arrays, and consultation in order to generate preliminary data to support the applicants RO1 proposal. Interested scientists should submit their research plan with a cover letter explaining how ECIS technology can be used to achieve their specific objectives. Applied BioPhysics will evaluate proposals based on scientific merit, suitability with ECIS technology and novelty.   



    To apply please send a resume, RO1 research plan and cover letter to Christian Renken at renken@biophysics.com.   

    ECIS Webinar Schedule 2013

    ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.

    All webinars are held at 11:00am EST. To register for a webinar, please go to:
    https://appliedbiophysics.webex.com and scroll to the webinar date of interest.    

    Cell Attachment and Spreading Measurements - 11:00 AM EST
    July 23 2013 


    Signal Transduction Assays - 11:00 AM EST
    September 10, 2013


    Toxicology with ECIS - 11:00 AM EST
    September 24, 2013


    ECIS Theory - 11:00 AM EST
    October 8, 2013


    Cell Invasion / Extravasation Assays - 11:00 AM EST
    October 22, 2013

    For a more detailed description of each webinar, please visit: http://www.biophysics.com/webinar.php

    New Publications



    Rasip1 mediates Rap1 regulation of Rho in endothelial barrier function through ArhGAP29 Anneke Post, Willem-Jan Pannekoek, Sarah H. Ross, Ingrid Verlaan, Patricia M. Brouwer, and Johannes L. Bos PNAS. published 24 June 2013, 10.1073/pnas.1306595110


    Embryonic Carcinoma Cells Show Specific Dielectric Resistance Profiles During Induced Differentiation

    Öz S, Maercker C, Breiling A. PLoS One. 2013;8(3):e59895. doi: 10.1371/journal.pone.0059895. Epub 2013 Mar 22


    Alcohol increases the permeability of airway epithelial tight junctions in Beas-2B and NHBE cells. Samantha M Simet, Todd A Wyatt, Jane DeVasure, Daniel Yanov, Diane Allen-Gipson, Joseph H Sisson Alcoholism Clinical and Experimental Research (impact factor: 3.34). 09/2011; 36(3):432-42. DOI:10.1111/j.1530-0277.2011.01640.x pp.432-42


    Src binds cortactin through an SH2 domain cystine-mediated linkage Jason V. Evans, Amanda G. Ammer, John E. Jett, Chris A. Bolcato, Jason C. Breaux, Karen H. Martin, Mark V. Culp, Peter M. Gannett, and Scott A. Weed
    J. Cell Sci. 2012; 125:6185-6197

    STIM1 Controls Endothelial Barrier Function Independently of Orai1 and Ca2+ Entry Arti V. Shinde, Rajender K. Motiani, Xuexin Zhang, Iskandar F. Abdullaev, Alejandro P. Adam, José C. González-Cobos, Wei Zhang, Khalid Matrougui, Peter A. Vincent, and Mohamed Trebak Sci. Signal. 2013; 6:ra18

    Central role of the exchange factor GEF-H1 in TNF-α-induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells Faiza Waheed, Qinghong Dan, Yasaman Amoozadeh, Yuqian Zhang, Susumu Tanimura, Pam Speight, András Kapus, and Katalin Szászi
    Mol. Biol. Cell. 2013; 24:1068-1082

    Neutrophil Proteinase 3 Acts on Protease-Activated Receptor-2 to Enhance Vascular Endothelial Cell Barrier Function Christopher J. Kuckleburg and Peter J. Newman Arterioscler Thromb Vasc Biol. 2013; 33:275-284

    Microparticles from Mycobacteria-Infected Macrophages Promote Inflammation and Cellular Migration
    Shaun B. Walters, Jens Kieckbusch, Gayathri Nagalingam, Ashleigh Swain, Sharissa L. Latham, Georges E. R. Grau, Warwick J. Britton, Valéry Combes, and Bernadette M. Saunders J. Immunol. 2013; 190:669-677  

    Knocking down Suppressor of Cytokine Signaling 7 in breast cancer: The role in Insulin-like Growth Factor - I/Phospholipase Cϒ-1 signaling W Sasi, L Ye, WG Jiang, K Mokbel, and A Sharma Cancer Res. 2012; 72:P1-04-03


    Nicotine Levels in Electronic Cigarettes Maciej L. Goniewicz, Tomasz Kuma, Michal Gawron, Jakub Knysak, and Leon Kosmider Nicotine Tob Res. 2013; 15:158-166

    VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx
    Siu P. Ngok, Rory Geyer, Miaoliang Liu, Antonis Kourtidis, Sudesh Agrawal, Chuanshen Wu, Himabindu Reddy Seerapu, Laura J. Lewis-Tuffin, Karen L. Moodie, Deborah Huveldt, Ruth Marx, Jay M. Baraban, Peter Storz, Arie Horowitz, and Panos Z. Anastasiadis J. Cell Biol. published 17 December 2012, 10.1083/jcb.201207009  


    P21 Activated Kinase-1 (Pak1) Promotes Prostate Tumor Growth and Microinvasion via Inhibition of Transforming Growth Factor β Expression and Enhanced Matrix Metalloproteinase 9 Secretion Anna Goc, Ahmad Al-Azayzih, Maha Abdalla, Belal Al-Husein, Sravankumar Kavuri, Jeffrey Lee, Kelvin Moses, and Payaningal R. Somanath J. Biol. Chem. 2013; 288:3025-3035 

    ADAM15 deficiency attenuates pulmonary hyperpermeability and acute lung injury in lipopolysaccharide-treated mice Chongxiu Sun, Richard S. Beard, Jr., Danielle L. McLean, Robert R. Rigor, Thomas Konia, Mack H. Wu, and Sarah Y. Yuan Am J Physiol Lung Cell Mol Physiol. 2013; 304:L135-L142

    NADPH Oxidase and Nrf2 Regulate Gastric Aspiration-Induced Inflammation and Acute Lung Injury
    Bruce A. Davidson, R. Robert Vethanayagam, Melissa J. Grimm, Barbara A. Mullan, Krishnan Raghavendran, Timothy S. Blackwell, Michael L. Freeman, Vanniarajan Ayyasamy, Keshav K. Singh, Michael B. Sporn, Kiyoshi Itagaki, Carl J. Hauser, Paul R. Knight, and Brahm H. Segal J. Immunol. 2013; 190:1714-1724  


    Smoke Extract Impairs Adenosine Wound Healing Implications of Smoke-Generated Oxidant. Diane S. Allen-Gipson, Matthew C. Zimmerman, Hui Zhang, Glenda Castellanos, Jennifer K. O'Malley, Horacio Alvarez-Ramirez, Kusum Kharbanda6, Joseph H. Sisson, and Todd A. Wyatt. Am. J. Respir. Cell Mol. Biol. rcmb. 2011-0273OC. First published online January 31, 2013 a doi:10.1165/rcmb.2011-0273OC


    Histone deacetylase (HDAC) inhibition prevents and restores bacterial lipopolysaccharide (LPS)-induced paracellular hyper-permeability in human lung microvascular endothelial cells (HLMVEC) Atul D Joshi, Gagan Thangjam, Nektarios Barabutis, Mary Shaw, Vijay Patel, and John D Catravas FASEB J. 2013; 27:647.12


    Autocrine regulation of endothelial barrier integrity by CAP37 Elizabeth Fox, Anne Pereira, and Jonathan Reichner FASEB J. 2013; 27:646.13


    Role for A-kinase anchoring proteins in cigarette smoke-induced barrier dysfunction Anouk Oldenburger, Wilfred Poppinga, Fleur Kos, Wolter Rijks, Irene Heijink, Wim Timens, Herman Meurs, Harm Maarsingh, and Martina Schmidt FASEB J. 2013; 27:1107.6

    Localized RhoA GTPase activity regulates dynamics of endothelial monolayer integrity Robert Szulcek, Cora M.L. Beckers, Jasmina Hodzic, Jelle de Wit, Zhenlong Chen, Tim Grob, Rene J.P. Musters, Richard D. Minshall, Victor W.M. van Hinsbergh, and Geerten P. van Nieuw Amerongen Cardiovasc Res. published 19 April 2013, 10.1093/cvr/cvt075  


    MicroRNAs Regulate Human Brain Endothelial Cell-Barrier Function in Inflammation: Implications for Multiple Sclerosis Arie Reijerkerk, M. Alejandro Lopez-Ramirez, Bert van het Hof, Joost A.R. Drexhage, Wouter W. Kamphuis, Gijs Kooij, Joost B. Vos, Tineke C.T.M. van der Pouw Kraan, Anton J. van Zonneveld, Anton J. Horrevoets, Alexandre Prat, Ignacio A. Romero, and Helga E. de Vries J. Neurosci. 2013; 33:6857-6863  


    A hemolytic pigment of Group B Streptococcus allows bacterial penetration of human placenta Christopher Whidbey, Maria Isabel Harrell, Kellie Burnside, Lisa Ngo, Alexis K. Becraft, Lakshminarayan M. Iyer, L. Aravind, Jane Hitti, Kristina M. Adams Waldorf, and Lakshmi Rajagopal J. Exp. Med. published 27 May 2013, 10.1084/jem.20122753  


    Glucocorticoid Induction of Occludin Expression and Endothelial Barrier Requires Transcription Factor p54 NONO Jason M. Keil, Xuwen Liu, and David A. Antonetti Invest. Ophthalmol. Vis. Sci. published 2 May 2013, 10.1167/iovs.13-11980   



    Have you recently published an article that includes the use of ECIS?
    If so, submit your publications to Applied BioPhysics via email to Dr. Christian Renken at renken@biophysics.com. We will announce your article in our newsletter, post it on our website and send you 
    two FREE 8 well arrays! 


    Upcoming Events

    Representatives from Applied BioPhysics will be at the following tradeshows and events:    



    University of Pittsburgh
    July 10, 2013
    Pittsburgh, PA


    IBCA 2013 
    August 21 - 23, 2013

    Budapest, Hungary


    Oct. 20 - 24, 2013

    Cape Cod, MA


    American Society for Cell Biology
    Dec. 14 - 18, 2013

    New Orleans, LA

     Tip of the Month:  



    ECIS® Array inoculation


    Careful inoculation is the key to good reproducibility from well to well and from experiment to experiment. It is most important to obtain a uniform inoculation of the well bottom. To accomplish this, wells should receive an even "snowfall" of cells so that each electrode has approximately the same number of cells falling on its surface - this can be facilitated by attentively following some important guidelines:

    1. Make up a mono-disperse cell suspension


    For some cell lines this is easily achieved, but for others, particularly if cells have been attached and spread for long periods of time, clumping takes place and longer trypsinization may be needed. The goal is to achieve is a clearly mono-disperse suspension of single cells.


    2. Keep the suspension agitated


    It is important to keep the cell suspension uniform so each well receives approximately the same number of cells; agitate the cell suspension frequently to prevent settling of the cells.

    3. Add the suspension to an empty well


    If possible, avoid mixing the cell suspension with liquid already in the well. It is best to remove all media from wells before adding the cell suspension such that no mixing within the wells is required. If a cell suspension must be added to liquid already in the well, thorough mixing of the two solutions is essential.

    4. Avoid unwanted convection during cell settling and attachment


    Temperature considerations are often overlooked and can be extremely important when dealing with cell distribution in small wells.


    If the temperature of the cell suspension is lower than that of the incubator, when the array is placed in the incubator, the wells will be heated from the bottom. This will cause a convection cell to form, where medium rises in the center and falls back down the walls of wells. Due to this flow, as cells attempt to fall to the central region of the well, they are swept upward. The overall effect is that the cell density becomes reduced in the central regions of the well. This is very undesirable especially with the 1E arrays and 10E arrays with their centrally located measuring electrodes. 





    Here are two ways to deal with this problem


    1) Simply inoculate the ECIS arrays outside of the incubator using medium at (or warmer than) room temperature and then wait 20 to 30 minutes before placing the array in the incubator space. Since there is no heating from below, there is no thermal convection as the cells settle over the substrate and begin to attach to the surface. We recently started using this approach, and the results have been most satisfactory. CO2 dependent medium would experience some pH increase out of the incubator in this protocol and one could consider ways to avoid this.   In our hands, however, we have not found a pH drift to be a problem, as once in the incubator space, we observe normal cell attachment and spreading impedance data.


    2) Alternatively, if solution #1 cannot be used, we then recommend using a cell suspension and array that are pre-warmed to incubator temperature and immediately place the array into the incubator upon inoculation. The goal again is to prevent thermal convection by having no heating of the wells taking place during cell settling.




    ECIS Humor

    Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.

    Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!

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