Now available from Springer Publishing  

Available Formats: e-Book $119.20 & Hardcover $151.20 

About this book

The first book devoted to cell based impedance sensing

Content Level » Research

Keywords »Cancer - Cell biology - Cell signalling - Impedance Sensing - Metastasis

Related subjects »Biomedical Sciences - Cancer Research - Molecular Medicine - Oncology & Hematology

Table of Contents

Preface.

1. Electric Cell-substrate Impedance Sensing Concept to Commercialisation. 

2. Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell    Impedance. 

3. ECIS as a tool in the study of metastasis suppressor genes: Epithelial Protein Lost In Neoplasm   (EPLIN).

4. Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening.

5. Epithelial-mesenchymal transition and the use of ECIS.

6. Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing.

7. Tight Junctions in Cancer Metastasis and their investigation using ECIS (Electric Cell-substrate Impedance Sensing).

8. Epithelial wound healing and the effects of cytokines investigated by ECIS.

9. Tumour-endothelial and tumour-mesothelial interactions investigated by impedance sensing based cell analyses.

10. Application of Electric Cell-Substrate Impedance Sensing in evaluation of traditional medicine on the cellular functions of gastric and colorecctal cancer cells.

11. Electric cell-substrate impedance sensing as a screening tool for wound healing agents.

12. ECIS, cellular adhesion and migration in keratinocytes. 13. Current and future application of ECIS models to study bone metastasis.

Index. 

Rasip1 mediates Rap1 regulation of Rho in endothelial barrier function through ArhGAP29 Anneke Post, Willem-Jan Pannekoek, Sarah H. Ross, Ingrid Verlaan, Patricia M. Brouwer, and Johannes L. Bos PNAS. published 24 June 2013, 10.1073/pnas.1306595110

Embryonic Carcinoma Cells Show Specific Dielectric Resistance Profiles During Induced Differentiation

Öz S, Maercker C, Breiling A. PLoS One. 2013;8(3):e59895. doi: 10.1371/journal.pone.0059895. Epub 2013 Mar 22

Alcohol increases the permeability of airway epithelial tight junctions in Beas-2B and NHBE cells. Samantha M Simet, Todd A Wyatt, Jane DeVasure, Daniel Yanov, Diane Allen-Gipson, Joseph H Sisson Alcoholism Clinical and Experimental Research (impact factor: 3.34). 09/2011; 36(3):432-42. DOI:10.1111/j.1530-0277.2011.01640.x pp.432-42

Src binds cortactin through an SH2 domain cystine-mediated linkage Jason V. Evans, Amanda G. Ammer, John E. Jett, Chris A. Bolcato, Jason C. Breaux, Karen H. Martin, Mark V. Culp, Peter M. Gannett, and Scott A. Weed
J. Cell Sci. 2012; 125:6185-6197

STIM1 Controls Endothelial Barrier Function Independently of Orai1 and Ca2+ Entry Arti V. Shinde, Rajender K. Motiani, Xuexin Zhang, Iskandar F. Abdullaev, Alejandro P. Adam, José C. González-Cobos, Wei Zhang, Khalid Matrougui, Peter A. Vincent, and Mohamed Trebak Sci. Signal. 2013; 6:ra18

Central role of the exchange factor GEF-H1 in TNF-α-induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells Faiza Waheed, Qinghong Dan, Yasaman Amoozadeh, Yuqian Zhang, Susumu Tanimura, Pam Speight, András Kapus, and Katalin Szászi
Mol. Biol. Cell. 2013; 24:1068-1082

Neutrophil Proteinase 3 Acts on Protease-Activated Receptor-2 to Enhance Vascular Endothelial Cell Barrier Function Christopher J. Kuckleburg and Peter J. Newman Arterioscler Thromb Vasc Biol. 2013; 33:275-284

Microparticles from Mycobacteria-Infected Macrophages Promote Inflammation and Cellular Migration
Shaun B. Walters, Jens Kieckbusch, Gayathri Nagalingam, Ashleigh Swain, Sharissa L. Latham, Georges E. R. Grau, Warwick J. Britton, Valéry Combes, and Bernadette M. Saunders J. Immunol. 2013; 190:669-677  
 

Knocking down Suppressor of Cytokine Signaling 7 in breast cancer: The role in Insulin-like Growth Factor - I/Phospholipase Cϒ-1 signaling W Sasi, L Ye, WG Jiang, K Mokbel, and A Sharma Cancer Res. 2012; 72:P1-04-03

Nicotine Levels in Electronic Cigarettes Maciej L. Goniewicz, Tomasz Kuma, Michal Gawron, Jakub Knysak, and Leon Kosmider Nicotine Tob Res. 2013; 15:158-166
 

VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx
Siu P. Ngok, Rory Geyer, Miaoliang Liu, Antonis Kourtidis, Sudesh Agrawal, Chuanshen Wu, Himabindu Reddy Seerapu, Laura J. Lewis-Tuffin, Karen L. Moodie, Deborah Huveldt, Ruth Marx, Jay M. Baraban, Peter Storz, Arie Horowitz, and Panos Z. Anastasiadis J. Cell Biol. published 17 December 2012, 10.1083/jcb.201207009  

P21 Activated Kinase-1 (Pak1) Promotes Prostate Tumor Growth and Microinvasion via Inhibition of Transforming Growth Factor β Expression and Enhanced Matrix Metalloproteinase 9 Secretion Anna Goc, Ahmad Al-Azayzih, Maha Abdalla, Belal Al-Husein, Sravankumar Kavuri, Jeffrey Lee, Kelvin Moses, and Payaningal R. Somanath J. Biol. Chem. 2013; 288:3025-3035 
 

ADAM15 deficiency attenuates pulmonary hyperpermeability and acute lung injury in lipopolysaccharide-treated mice Chongxiu Sun, Richard S. Beard, Jr., Danielle L. McLean, Robert R. Rigor, Thomas Konia, Mack H. Wu, and Sarah Y. Yuan Am J Physiol Lung Cell Mol Physiol. 2013; 304:L135-L142
 

NADPH Oxidase and Nrf2 Regulate Gastric Aspiration-Induced Inflammation and Acute Lung Injury
Bruce A. Davidson, R. Robert Vethanayagam, Melissa J. Grimm, Barbara A. Mullan, Krishnan Raghavendran, Timothy S. Blackwell, Michael L. Freeman, Vanniarajan Ayyasamy, Keshav K. Singh, Michael B. Sporn, Kiyoshi Itagaki, Carl J. Hauser, Paul R. Knight, and Brahm H. Segal J. Immunol. 2013; 190:1714-1724  

Smoke Extract Impairs Adenosine Wound Healing Implications of Smoke-Generated Oxidant. Diane S. Allen-Gipson, Matthew C. Zimmerman, Hui Zhang, Glenda Castellanos, Jennifer K. O'Malley, Horacio Alvarez-Ramirez, Kusum Kharbanda6, Joseph H. Sisson, and Todd A. Wyatt. Am. J. Respir. Cell Mol. Biol. rcmb. 2011-0273OC. First published online January 31, 2013 a doi:10.1165/rcmb.2011-0273OC

Histone deacetylase (HDAC) inhibition prevents and restores bacterial lipopolysaccharide (LPS)-induced paracellular hyper-permeability in human lung microvascular endothelial cells (HLMVEC) Atul D Joshi, Gagan Thangjam, Nektarios Barabutis, Mary Shaw, Vijay Patel, and John D Catravas FASEB J. 2013; 27:647.12

Autocrine regulation of endothelial barrier integrity by CAP37 Elizabeth Fox, Anne Pereira, and Jonathan Reichner FASEB J. 2013; 27:646.13

Role for A-kinase anchoring proteins in cigarette smoke-induced barrier dysfunction Anouk Oldenburger, Wilfred Poppinga, Fleur Kos, Wolter Rijks, Irene Heijink, Wim Timens, Herman Meurs, Harm Maarsingh, and Martina Schmidt FASEB J. 2013; 27:1107.6
 

Localized RhoA GTPase activity regulates dynamics of endothelial monolayer integrity Robert Szulcek, Cora M.L. Beckers, Jasmina Hodzic, Jelle de Wit, Zhenlong Chen, Tim Grob, Rene J.P. Musters, Richard D. Minshall, Victor W.M. van Hinsbergh, and Geerten P. van Nieuw Amerongen Cardiovasc Res. published 19 April 2013, 10.1093/cvr/cvt075  

MicroRNAs Regulate Human Brain Endothelial Cell-Barrier Function in Inflammation: Implications for Multiple Sclerosis Arie Reijerkerk, M. Alejandro Lopez-Ramirez, Bert van het Hof, Joost A.R. Drexhage, Wouter W. Kamphuis, Gijs Kooij, Joost B. Vos, Tineke C.T.M. van der Pouw Kraan, Anton J. van Zonneveld, Anton J. Horrevoets, Alexandre Prat, Ignacio A. Romero, and Helga E. de Vries J. Neurosci. 2013; 33:6857-6863  

A hemolytic pigment of Group B Streptococcus allows bacterial penetration of human placenta Christopher Whidbey, Maria Isabel Harrell, Kellie Burnside, Lisa Ngo, Alexis K. Becraft, Lakshminarayan M. Iyer, L. Aravind, Jane Hitti, Kristina M. Adams Waldorf, and Lakshmi Rajagopal J. Exp. Med. published 27 May 2013, 10.1084/jem.20122753  

Glucocorticoid Induction of Occludin Expression and Endothelial Barrier Requires Transcription Factor p54 NONO Jason M. Keil, Xuwen Liu, and David A. Antonetti Invest. Ophthalmol. Vis. Sci. published 2 May 2013, 10.1167/iovs.13-11980   

ECIS® Array inoculation

Careful inoculation is the key to good reproducibility from well to well and from experiment to experiment. It is most important to obtain a uniform inoculation of the well bottom. To accomplish this, wells should receive an even "snowfall" of cells so that each electrode has approximately the same number of cells falling on its surface - this can be facilitated by attentively following some important guidelines:
 

2. Keep the suspension agitated

It is important to keep the cell suspension uniform so each well receives approximately the same number of cells; agitate the cell suspension frequently to prevent settling of the cells.

 3. Add the suspension to an empty well

If possible, avoid mixing the cell suspension with liquid already in the well. It is best to remove all media from wells before adding the cell suspension such that no mixing within the wells is required. If a cell suspension must be added to liquid already in the well, thorough mixing of the two solutions is essential.

 4. Avoid unwanted convection during cell settling and attachment

Temperature considerations are often overlooked and can be extremely important when dealing with cell distribution in small wells.

If the temperature of the cell suspension is lower than that of the incubator, when the array is placed in the incubator, the wells will be heated from the bottom. This will cause a convection cell to form, where medium rises in the center and falls back down the walls of wells. Due to this flow, as cells attempt to fall to the central region of the well, they are swept upward. The overall effect is that the cell density becomes reduced in the central regions of the well. This is very undesirable especially with the 1E arrays and 10E arrays with their centrally located measuring electrodes. 

Here are two ways to deal with this problem

1) Simply inoculate the ECIS arrays outside of the incubator using medium at (or warmer than) room temperature and then wait 20 to 30 minutes before placing the array in the incubator space. Since there is no heating from below, there is no thermal convection as the cells settle over the substrate and begin to attach to the surface. We recently started using this approach, and the results have been most satisfactory. CO2 dependent medium would experience some pH increase out of the incubator in this protocol and one could consider ways to avoid this.   In our hands, however, we have not found a pH drift to be a problem, as once in the incubator space, we observe normal cell attachment and spreading impedance data.

2) Alternatively, if solution #1 cannot be used, we then recommend using a cell suspension and array that are pre-warmed to incubator temperature and immediately place the array into the incubator upon inoculation. The goal again is to prevent thermal convection by having no heating of the wells taking place during cell settling.