The ECIS Connection - March 2013              


ECIS Training Schedule


May 9 & 10, 2013


Applied BioPhysics is offering a two day in depth ECIS training course. The course will include both lecture and wet lab training. The training will take place at the Applied BioPhysics facility in Troy, NY. Training will be conducted by Dr. Charles Keese, Dr. Ivar Giaever, Dr. Christian Renken, Dr. Judith Stolwijk and Catherine Toniatti-Yanulavich.


Attendance will be limited to a maximum of 6 people.


Cost is $1,250 per person. This includes two nights of lodging, transportation to and from the hotel to the Applied BioPhysics facility, lunch on both days and dinner Thursday evening. To register, contact Wendy Ladouceur for a registration form at 518-880-6860, ladouceur@biophysics.com or download the form from our website, biophysics.com.


ECIS-related topics to be covered include: 

  • Experimental design and array selection
  • Array preparation and stabilization
  • Obtaining good well-to-well and experiment-to-experiment repeatability
  • Applying extracellular matrix proteins to the electrodes
  • Techniques for array inoculation
  • Techniques for addition of compounds to ECIS wells
  • Basics of the ECIS software for data acquisition
  • Advanced features of the ECIS software for data acquisition
  • Basics of the data analysis software
  • Advanced features of the data analysis software
  • Basics of impedance measurements
  • Theory behind ECIS
  • Simple and complex impedance and the value of R and C in cell measurements
  • Selecting the AC frequency or frequencies for experiments
  • Modeling ECIS data
  • In situ electroporation
  • Cell migration measurements with the ECIS "wound-healing" assay
  • Cell migration measurements with the "electric fence"
  • A survey of cell biology applications using ECIS
  • Fluorescence staining of cells on the ECIS array 


Now available from Springer Publishing  


Electric Cell-Substrate Impedance Sensing and Cancer Metastasis

Jiang, Wen G. (Ed.)

2012, VIII, 260 p. 76 illus., 60 in color.



 Available Formats: e-Book $119.20 & Hardcover $151.20


ECIS Book 


About this book


The first book devoted to cell based impedance sensing

  • Written by experts in this area
  • Highly informative colour figures
  • Protocols and methods

    Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.

    Content Level » Research

    Keywords »Cancer - Cell biology - Cell signalling - Impedance Sensing - Metastasis

    Related subjects »Biomedical Sciences - Cancer Research - Molecular Medicine - Oncology & Hematology


    Table of Contents



    1. Electric Cell-substrate Impedance Sensing Concept to Commercialisation. 

    2. Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell    Impedance. 

    3. ECIS as a tool in the study of metastasis suppressor genes: Epithelial Protein Lost In Neoplasm   (EPLIN).

    4. Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening.

    5. Epithelial-mesenchymal transition and the use of ECIS.

    6. Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing.

    7. Tight Junctions in Cancer Metastasis and their investigation using ECIS (Electric Cell-substrate Impedance Sensing).

    8. Epithelial wound healing and the effects of cytokines investigated by ECIS.

    9. Tumour-endothelial and tumour-mesothelial interactions investigated by impedance sensing based cell analyses.

    10. Application of Electric Cell-Substrate Impedance Sensing in evaluation of traditional medicine on the cellular functions of gastric and colorecctal cancer cells.

    11. Electric cell-substrate impedance sensing as a screening tool for wound healing agents.

    12. ECIS, cellular adhesion and migration in keratinocytes. 13. Current and future application of ECIS models to study bone metastasis.









    Wednesday August 21, 2013 at 12:30 PM CEST
    Friday August 23, 2013 at 12:30 PM CEST
    Add to Calendar



    Semmelweis University, Budapest, Hungary
    Nagyvarad ter 4.
    Budapest H-1089

    Driving Directions











    On behalf of the Scientific Committee and myself I would like to invite you to the 2nd International Conference on Impedance Based Cellular Assays in Budapest, Hungary on August 21 - 23.


    Nearly two years have passed since the first IBCA meeting that brought together people using electrical impedance measurements to study cells in vitro. Our aim is to link together the growing number of researchers in this area, to share ideas and protocols and to discuss future directions of the technology.


    As the host of IBCA2013 I would like to invite you and your colleagues who have an interest in non-invasive cell monitoring to join us and to be part of this year's meeting. Please save the date of August 21st to 23rd.


    The beautiful city of Budapest will hopefully set the stage for another round of inspiring presentations and fruitful discussions about this most versatile way of whole-cell biosensing.


    I look forward to welcome you all in Budapest for IBCA2013


    Laszlo Kohidai 



    Register Now!

    I can't make it


    Scientific Committee

    Laszlo Kohidai (Chair)                            Semmelweis University

    Nikolaj Gadegaard                                    University of Glasgow

    Hamed Laroui                                      Georgia State University

    Chun-Min Lo                                National Yang-Ming University

    Geerten van Nieuw Amerongen     Free University Medical Center

    Mohamed Trebak                                        University of Albany

    Joachim Wegener                                University of Regensburg




    ECIS Software   


    Latest Software version:

    The latest version of the ECIS software is v1.2.123 and available for download from:




    New functionality - Electric Fence



    This update of the software incorporates the official release of the "Electric Fence" which is available on systems that have the Wounding/Electroporate capability (EFM option).


    The Electric Fence (EF) applies a short pulse to the selected electrodes on a regular basis during data collection (e.g. every 5 minutes). This prevents the cells from growing onto the electrodes. When the layer is confluent the electric fence can be turned off and the rate of cell migration onto the empty electrodes can be monitored. This is particularly useful with cell types that may resist the normal wounding mechanism as the cells never have a chance to attach to the electrode. This also means that the data collection should begin and the electric fence applied as soon as the cells are added to the wells.


    The Electric Fence setup is available from the Wound/Electroporate options. The desired wells should first be selected, then click Electric Fence. The default time interval is 5 minutes, and the EF will be applied after a full time point is collected. The frequency and current levels have been preset for the selected well type. Note that normal Wounding/Electroporate functionality and Pausing are still available while the EF is on, but these will temporarily interrupt the EF application. 


     Early Career Mini-Grants


    Mini Grant Array Image  


    Applied BioPhysics would like to help young scientists obtain funding. The ECIS mini-grant is aimed at early career scientists who are applying for their first RO1 grant. For a researcher wanting to use ECIS technology to achieve their research goals, Applied BioPhysics will provide an ECIS instrument, ECIS arrays, and consultation in order to generate preliminary data to support the applicants RO1 proposal. Interested scientists should submit their research plan with a cover letter explaining how ECIS technology can be used to achieve their specific objectives. Applied BioPhysics will evaluate proposals based on scientific merit, suitability with ECIS technology and novelty.




    To apply please send a resume, RO1 research plan and cover letter to Christian Renken at renken@biophysics.com



    ECIS Webinar Schedule 2012

    ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.

    All webinars are held at 11:00am EST. To register for a webinar, please go to:
    https://appliedbiophysics.webex.com and scroll to the webinar date of interest.    



    Cell Attachment and Spreading Measurements - 11:00 AM EST
    March 19, 2013


    Signal Transduction Assays - 11:00 AM EST
    April 2, 2013


    Toxicology with ECIS - 11:00 AM EST
    April 16, 2013


    ECIS Theory - 11:00 AM EST
    May 7, 2013

    Cell Invasion / Extravasation Assays - 11:00 AM EST
    May 21, 2013 


    Automated Cell Migration - 11:00 AM EST 
    June 4, 2013

    Barrier Function Assays - 11:00 AM EST
    June 18, 2013 


    Real-time Electroporation and Monitoring - 11:00 AM EST
    July 9, 2013


    Cell Attachment and Spreading Measurements - 11:00 AM EST
    July 23 2013 

    For a more detailed description of each webinar, please visit: http://www.biophysics.com/webinar.php


    New 96 well array is shipping!




    The " 96W20idf PET"  array  utilizes a completely new interface of pogo pins to our  ECIS 96 well station. The gold electrode is located on 10mil thick PET material  and optically clear so cells can be viewed on an inverted microscope. The gold electrode design is an inter-digitated finger arrangement . It has twice the area of our standard 10E+ electrodes.


    New Publications



    Retinoic Acid Induces Blood-Brain Barrier Development Mark R. Mizee, Desiree Wooldrik, Kim A. M. Lakeman, Bert van het Hof, Joost A. R. Drexhage, Dirk Geerts, Marianna Bugiani, Eleonora Aronica, Reina E. Mebius, Alexandre Prat, Helga E. de Vries, and Arie Reijerkerk J. Neurosci. 2013; 33:1660-1671.  

    MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
    Juan F. Aranda, Natalia Reglero-Real, Beatriz Marcos-Ramiro, Ana Ruiz-Sáenz, Laura Fernández-Martín, Miguel Bernabé-Rubio, Leonor Kremer, Anne J. Ridley, Isabel Correas, Miguel A. Alonso, and Jaime Millán
    Mol. Biol. Cell. 2013; 24:483-494 


    COM-1/p8 Acts as a Tumour Growth Enhancer in Colorectal Cancer Cell Lines XIN LI, TRACEY A. MARTIN, and WEN G. JIANG Anticancer Res. 2012; 32:1229-1237.


    EPLIN-α Expression in Human Oesophageal Cancer and its Impact on Cellular Aggressiveness and Clinical Outcome YINAN LIU, ANDREW J. SANDERS, LIJIAN ZHANG, and WEN G. JIANG Anticancer Res. 2012; 32:1283-1289. 

    Impact of Yangzheng Xiaoji on the Adhesion and Migration of Human Cancer Cells: the Role of the AKT Signalling Pathway L Ye, K Ji, N Frewer, J Ji, and WG Jiang Anticancer Res. 2012; 32:2537.


    Loss of Expression of Growth Differentiation Factor-9 (GDF9) in Human Kidney Cancer and Regulation of Growth and Migration of Kidney Cancer Cells by GDF9 PENG DU, LIN YE, HAN LI, FIONA RUGE, YONG YANG, and WEN G. JIANG Anticancer Res. 2012; 32:4375-4383.


    Ezrin, Radixin, and Moesin Are Phosphorylated in Response to 2-Methoxyestradiol and Modulate Endothelial Hyperpermeability (Feb 24) Natalia V. Bogatcheva, Marina A. Zemskova, Boris A. Gorshkov, Kyung Mi Kim, Gregory A. Daglis, Christophe Poirier, and Alexander D. Verin Am. J. Respir. Cell Mol. Biol. 2011; 45:1185-1194.

    Bacillus anthracis Lethal Toxin Reduces Human Alveolar Epithelial Barrier Function Marybeth Langer, Elizabeth Stewart Duggan, John Leland Booth, Vineet Indrajit Patel, Ryan A. Zander, Robert Silasi-Mansat, Vijay Ramani, Tibor Zoltan Veres, Frauke Prenzler, Katherina Sewald, Daniel M. Williams, Kenneth Mark Coggeshall, Shanjana Awasthi, Florea Lupu, Dennis Burian, Jimmy Dale Ballard, Armin Braun, and Jordan Patrick Metcalf Infect. Immun. 2012; 80:4374-4387


    The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation Jos van Rijssel, Jeffrey Kroon, Mark Hoogenboezem, Floris P. J. van
    Alphen, Renske J. de Jong, Elena Kostadinova, Dirk Geerts, Peter L. Hordijk, and Jaap D. van Buul Mol. Biol. Cell. 2012; 23:2831-2844.


    Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts Micha Nethe, Bart-Jan de Kreuk, Daniele V. F. Tauriello, Eloise C. Anthony, Barbara Snoek, Thomas Stumpel, Patricia C. Salinas, Madelon M. Maurice, Dirk Geerts, André M. Deelder, Paul J. Hensbergen, and Peter L. Hordijk J. Cell Sci. 2012; 125:3430-3442.


    The tyrosine phosphatase SHP2 regulates recovery of endothelial adherens junctions through control of β-catenin phosphorylation Ilse Timmerman, Mark Hoogenboezem, Anton M. Bennett, Dirk Geerts, Peter L. Hordijk, and Jaap D. van Buul Mol. Biol. Cell. 2012; 23:4212-4225.


    p120-Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylation Pilar Alcaide, Roberta Martinelli, Gail Newton, Marcie R. Williams,
    Alejandro Adam, Peter A. Vincent, and Francis W. Luscinskas Am J Physiol Cell Physiol. 2012; 303:C385-C395.


    Circulating Factors Induced by Caloric Restriction in the Nonhuman Primate Macaca Mulatta Activate Angiogenic Processes in Endothelial Cells Anna Csiszar, Danuta Sosnowska, Zsuzsanna Tucsek, Tripti Gautam, Peter Toth, Gyorgy Losonczy, Ricki J. Colman, Richard Weindruch, Rozalyn M. Anderson, William E. Sonntag, and Zoltan Ungvari J Gerontol A Biol Sci Med Sci. published 17 August 2012, 10.1093/gerona/gls158


    The atypical chemokine receptor D6 controls macrophage efferocytosis and cytokine secretion during the resolution of inflammation Ester Pashover-Schallinger, Miran Aswad, Sagie Schif-Zuck, Haim Shapiro, Pierre Singer, and Amiram Ariel FASEB J. 2012; 26:3891-3900.


    PKCα Activation of p120-Catenin Serine 879 Phospho-Switch Disassembles VE-Cadherin Junctions and Disrupts Vascular Integrity Emily Vandenbroucke St Amant, Mohammad Tauseef, Stephen M. Vogel, Xiao-Pei Gao, Dolly Mehta, Yulia A. Komarova, and Asrar B. Malik Circ. Res. 2012; 111:739-749.


    Sustained Adenosine Exposure Causes Lung Endothelial Barrier Dysfunction via Nucleoside transporter-Mediated Signaling Qing Lu, Julie Newton, Vivian Hsiao, Paul Shamirian, Michael R. Blackburn, and Mesias Pedroza Am. J. Respir. Cell Mol. Biol. 2012;47:604-613

    Absence of Glial α-Dystrobrevin Causes Abnormalities of the Blood-Brain Barrier and Progressive Brain Edema Chun Fu Lien, Sarajo Kumar Mohanta, Malgorzata Frontczak-Baniewicz, Jerome D. Swinny, Barbara Zablocka, and Dariusz C. Górecki J. Biol. Chem. 2012; 287:41374-41385. 


    FGF-9 accelerates epithelial invagination for ectodermal organogenesis in real time bioengineered organ manipulation YY Tai, RS Chen, Y Lin, TY Ling, and MH Chen Cell Commun Signal. 2012; 10: 34. [MEDLINE Citation]

    Aging-Induced Dysregulation of Dicer1-Dependent MicroRNA Expression Impairs Angiogenic Capacity of Rat Cerebromicrovascular Endothelial Cells Zoltan Ungvari, Zsuzsanna Tucsek, Danuta Sosnowska, Peter Toth, Tripti Gautam, Andrej Podlutsky, Agnes Csiszar, Gyorgy Losonczy, M. Noa Valcarcel-Ares, William E. Sonntag, and Anna Csiszar J Gerontol A Biol Sci Med Sci. published 13 December 2012, 10.1093/gerona/gls242


    Sphingosine 1-Phosphate (S1P) Carrier-dependent Regulation of Endothelial Barrier: HIGH DENSITY LIPOPROTEIN (HDL)-S1P PROLONGS ENDOTHELIAL BARRIER ENHANCEMENT AS COMPARED WITH ALBUMIN-S1P VIA EFFECTS ON LEVELS, TRAFFICKING, AND SIGNALING OF S1P1 Brent A. Wilkerson, G. Daniel Grass, Shane B. Wing, W. Scott Argraves, and Kelley M. Argraves J. Biol. Chem. 2012; 287:44645-44653.


    High-mobility group box-1 protein activates inflammatory signaling pathway components and disrupts retinal vascular-barrier in the diabetic retina. Mohammad G, Siddiquei MM, Othman A, Al-Shabrawey M, Abu El-Asrar AM. Exp Eye Res. 2012 Dec 21;107C:101-109. doi: 10.1016/j.exer.2012.12.009. [Epub ahead of print] PMID: 23261684


    Arresten, a collagen-derived angiogenesis inhibitor, suppresses invasion of squamous cell carcinoma

    Aikio M, Alahuhta I, Nurmenniemi S, Suojanen J, Palovuori R, Teppo S, Sorsa T, López-Otín C, Pihlajaniemi T, Salo T, Heljasvaara R, Nyberg P. PLoS One. 2012;7(12):e51044. doi: 10.1371/journal.pone.0051044. Epub 2012 Dec 5.

    PMID: 23227231

    FGF-9 accelerates epithelial invagination for ectodermal organogenesis in real time bioengineered organ manipulation Tai YY, Chen RS, Lin Y, Ling TY, Chen MH. Cell Commun Signal. 2012 Nov 23;10(1):34. doi: 10.1186/1478-811X-10-34. PMID: 23176204


    A vertically aligned carbon nanotube-based impedance sensing biosensor for rapid and high sensitive detection of cancer cells Abdolahad M, Taghinejad M, Taghinejad H, Janmaleki M, Mohajerzadeh S. Lab Chip. 2012 Mar 21;12(6):1183-90. doi: 10.1039/c2lc21028b. Epub 2012 Jan 31. PMID: 22294045


     Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation Marimuthu M, Park C, Kim S, Choi CS. Int J Nanomedicine. 2012;7:83-92. doi: 10.2147/IJN.S28465. Epub 2012 Jan 6.
    PMID: 22275825


    A computational modeling and analysis in cell biological dynamics using electric cell-substrate impedance sensing (ECIS) Chen SW, Yang JM, Yang JH, Yang SJ, Wang JS. Biosens Bioelectron. 2012 Mar 15;33(1):196-203. doi: 10.1016/j.bios.2011.12.052. Epub 2012 Jan 3. PMID: 22261483


    Use of cellular electrical impedance sensing to assess in vitro cytotoxicity of anticancer drugs in a human kidney cell nephrotoxicity model Xie F, Xu Y, Wang L, Mitchelson K, Xing W, Cheng J. Analyst. 2012 Mar 21;137(6):1343-50. doi: 10.1039/c2an16141a. Epub 2012 Jan 12. PMID: 22214987 

    A microfluidic device with passive air-bubble valves for real-time measurement of dose-dependent drug cytotoxicity through impedance sensing Xu Y, Lv Y, Wang L, Xing W, Cheng J. Biosens Bioelectron. 2012 Feb 15;32(1):300-4. doi: 10.1016/j.bios.2011.12.009. Epub 2011 Dec 13. PMID: 22208957

    Neuronal markers are expressed in human gliomas and NSE knockdown sensitizes glioblastoma cells to radiotherapy and temozolomide Yan T, Skaftnesmo KO, Leiss L, Sleire L, Wang J, Li X, Enger PŘ. BMC Cancer. 2011 Dec 20;11:524. doi: 10.1186/1471-2407-11-524.



    Have you recently published an article that includes the use of ECIS?
    If so, submit your publications to Applied BioPhysics via email to Dr. Christian Renken at renken@biophysics.com. We will announce your article in our newsletter, post it on our website and send you 
    two FREE 8 well arrays! 
    Upcoming Events

    Representatives from Applied BioPhysics will be at the following tradeshows and events:    



    Society of Toxicology
    March 10 - 14, 2013

    San Antonio, TX


    Cleveland Clinic

    March 12, 2013
    Cleveland, OH


    Case Western Reserve University
    March 13, 2013

    Cleveland, OH


    American Association for Cancer Research
    April 6 - 10, 2013
    Washington, DC 


    Experimental Biology
    April 20 - 24, 2013
    Boston, MA 


    American Thoracic Society International Conference
    May 17 - 22, 2013

    Philadelphia, PA


    10th International Conference on Cerebral Vascular Biology
    June 18 - 21, 2013

    Montreal, Quebec


    Washington University
    June 26, 2013
    St. Louis, MO 


    St. Louis University

    June 27, 2013
    St. Louis, MO


    University of Pittsburgh
    July 10, 2013

    Pittsburgh, PA


    IBCA 2013 
    August 21 - 23, 2013

    Budapest, Hungary


    Oct. 20 - 24, 2013

    Cape Cod, MA


    American Society for Cell Biology
    Dec. 14 - 18, 2013

    New Orleans, LA

    Tip of the Month:  



    Adding compounds to study their effect upon ECIS measurements 


    The ECIS measurement is extremely sensitive; even the responses of cells to small changes in temperature, shear stress, CO2 concentration, etc. are detectable. Because of this, care must be taken when adding compounds to wells to minimize unrelated responses and ensure that the biological effects of the compounds being added are clearly observed.

    The following is a suggested protocol:

    An hour before the addition of the compound, change the medium over the cells using the medium that will be present when the compound is added - often serum-free medium. This provides the cells time to adjust and equilibrate to the experimental conditions. Follow the equilibration process using ECIS to be certain enough time has elapsed to give a steady impedance signal.

    Prepare the compound to be tested at twice the final concentration (2X) in the same solution as that in the wells and allow the solution to equilibrate to incubator conditions. Next, withdraw one-half the volume of medium in a well and then add back this same volume of the 2X solution to achieve the final 1X concentration. The addition should be done slowly, so the cells on the active electrodes are not exposed to shear stress from the fluid flow. 

    If you are removing the array from the incubator for this addition, be sure to pause the measurement and work quickly to minimize temperature drops. Once the array is returned to the incubator, perform an electrode check to ensure contact has been reestablished and then immediately resume data collection.

    If the compound addition is carried out without removing the array from its holder, pausing the measurement during this addition is recommended to avoid any unwanted impedance spikes.

    Of course, be sure to always include a control, where all manipulations are carried out but without compound present.





      Ivar Ingar Skiing


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    Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!

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