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| The ECIS Connection - March 2013 |
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ECIS Training Schedule
May 9 & 10, 2013
Applied BioPhysics is offering a two day in depth ECIS training course. The course will include both lecture and wet lab training. The training will take place at the Applied BioPhysics facility in Troy, NY. Training will be conducted by Dr. Charles Keese, Dr. Ivar Giaever, Dr. Christian Renken, Dr. Judith Stolwijk and Catherine Toniatti-Yanulavich.
Attendance will be limited to a maximum of 6 people.
Cost is $1,250 per person. This includes two nights of lodging, transportation to and from the hotel to the Applied BioPhysics facility, lunch on both days and dinner Thursday evening. To register, contact Wendy Ladouceur for a registration form at 518-880-6860, ladouceur@biophysics.com or download the form from our website, biophysics.com.
ECIS-related topics to be covered include:
- Experimental design and array selection
- Array preparation and stabilization
- Obtaining good well-to-well and experiment-to-experiment repeatability
- Applying extracellular matrix proteins to the electrodes
- Techniques for array inoculation
- Techniques for addition of compounds to ECIS wells
- Basics of the ECIS software for data acquisition
- Advanced features of the ECIS software for data acquisition
- Basics of the data analysis software
- Advanced features of the data analysis software
- Basics of impedance measurements
- Theory behind ECIS
- Simple and complex impedance and the value of R and C in cell measurements
- Selecting the AC frequency or frequencies for experiments
- Modeling ECIS data
- In situ electroporation
- Cell migration measurements with the ECIS "wound-healing" assay
- Cell migration measurements with the "electric fence"
- A survey of cell biology applications using ECIS
- Fluorescence staining of cells on the ECIS array
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Now available from Springer Publishing
Electric Cell-Substrate Impedance Sensing and Cancer Metastasis
2012, VIII, 260 p. 76 illus., 60 in color.
Available Formats: e-Book $119.20 & Hardcover $151.20
About this book
The first book devoted to cell based impedance sensing
Written by experts in this area
Highly informative colour figures
Protocols and methods
Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.
Content Level » Research
Keywords »Cancer - Cell biology - Cell signalling - Impedance Sensing - Metastasis
Related subjects »Biomedical Sciences - Cancer Research - Molecular Medicine - Oncology & Hematology
Table of Contents
Preface.
1. Electric Cell-substrate Impedance Sensing Concept to Commercialisation.
2. Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell Impedance.
3. ECIS as a tool in the study of metastasis suppressor genes: Epithelial Protein Lost In Neoplasm (EPLIN).
4. Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening.
5. Epithelial-mesenchymal transition and the use of ECIS.
6. Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing.
7. Tight Junctions in Cancer Metastasis and their investigation using ECIS (Electric Cell-substrate Impedance Sensing).
8. Epithelial wound healing and the effects of cytokines investigated by ECIS.
9. Tumour-endothelial and tumour-mesothelial interactions investigated by impedance sensing based cell analyses.
10. Application of Electric Cell-Substrate Impedance Sensing in evaluation of traditional medicine on the cellular functions of gastric and colorecctal cancer cells.
11. Electric cell-substrate impedance sensing as a screening tool for wound healing agents.
12. ECIS, cellular adhesion and migration in keratinocytes. 13. Current and future application of ECIS models to study bone metastasis.
Index.
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When
Wednesday August 21, 2013 at 12:30 PM CEST
-to-
Friday August 23, 2013 at 12:30 PM CEST
Add to Calendar
Where
Semmelweis University, Budapest, Hungary
Nagyvarad ter 4.
Budapest H-1089
Hungary
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On behalf of the Scientific Committee and myself I would like to invite you to the 2nd International Conference on Impedance Based Cellular Assays in Budapest, Hungary on August 21 - 23.
Nearly two years have passed since the first IBCA meeting that brought together people using electrical impedance measurements to study cells in vitro. Our aim is to link together the growing number of researchers in this area, to share ideas and protocols and to discuss future directions of the technology.
As the host of IBCA2013 I would like to invite you and your colleagues who have an interest in non-invasive cell monitoring to join us and to be part of this year's meeting. Please save the date of August 21st to 23rd.
The beautiful city of Budapest will hopefully set the stage for another round of inspiring presentations and fruitful discussions about this most versatile way of whole-cell biosensing.
I look forward to welcome you all in Budapest for IBCA2013
Laszlo Kohidai
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Scientific Committee
Laszlo Kohidai (Chair) Semmelweis University
Nikolaj Gadegaard University of Glasgow
Hamed Laroui Georgia State University
Chun-Min Lo National Yang-Ming University
Geerten van Nieuw Amerongen Free University Medical Center
Mohamed Trebak University of Albany
Joachim Wegener University of Regensburg |
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ECIS Software
The latest version of the ECIS software is v1.2.123 and available for download from:
http://www.biophysics.com/software/ECIS_Software_v1_2_123.msi
New functionality - Electric Fence
This update of the software incorporates the official release of the "Electric Fence" which is available on systems that have the Wounding/Electroporate capability (EFM option).
The Electric Fence (EF) applies a short pulse to the selected electrodes on a regular basis during data collection (e.g. every 5 minutes). This prevents the cells from growing onto the electrodes. When the layer is confluent the electric fence can be turned off and the rate of cell migration onto the empty electrodes can be monitored. This is particularly useful with cell types that may resist the normal wounding mechanism as the cells never have a chance to attach to the electrode. This also means that the data collection should begin and the electric fence applied as soon as the cells are added to the wells.
The Electric Fence setup is available from the Wound/Electroporate options. The desired wells should first be selected, then click Electric Fence. The default time interval is 5 minutes, and the EF will be applied after a full time point is collected. The frequency and current levels have been preset for the selected well type. Note that normal Wounding/Electroporate functionality and Pausing are still available while the EF is on, but these will temporarily interrupt the EF application. |
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Early Career Mini-Grants
Applied BioPhysics would like to help young scientists obtain funding. The ECIS mini-grant is aimed at early career scientists who are applying for their first RO1 grant. For a researcher wanting to use ECIS technology to achieve their research goals, Applied BioPhysics will provide an ECIS instrument, ECIS arrays, and consultation in order to generate preliminary data to support the applicants RO1 proposal. Interested scientists should submit their research plan with a cover letter explaining how ECIS technology can be used to achieve their specific objectives. Applied BioPhysics will evaluate proposals based on scientific merit, suitability with ECIS technology and novelty.
To apply please send a resume, RO1 research plan and cover letter to Christian Renken at renken@biophysics.com.
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ECIS Webinar Schedule 2012
ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.
All webinars are held at 11:00am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com and scroll to the webinar date of interest. Cell Attachment and Spreading Measurements - 11:00 AM EST
March 19, 2013
Signal Transduction Assays - 11:00 AM EST
April 2, 2013
Toxicology with ECIS - 11:00 AM EST
April 16, 2013
ECIS Theory - 11:00 AM EST
May 7, 2013
Cell Invasion / Extravasation Assays - 11:00 AM EST
May 21, 2013
Automated Cell Migration - 11:00 AM EST
June 4, 2013
Barrier Function Assays - 11:00 AM EST
June 18, 2013
Real-time Electroporation and Monitoring - 11:00 AM EST
July 9, 2013
Cell Attachment and Spreading Measurements - 11:00 AM EST
July 23 2013
For a more detailed description of each webinar, please visit: http://www.biophysics.com/webinar.php
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New 96 well array is shipping!
The " 96W20idf PET" array utilizes a completely new interface of pogo pins to our ECIS 96 well station. The gold electrode is located on 10mil thick PET material and optically clear so cells can be viewed on an inverted microscope. The gold electrode design is an inter-digitated finger arrangement . It has twice the area of our standard 10E+ electrodes.
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New Publications
Retinoic Acid Induces Blood-Brain Barrier Development Mark R. Mizee, Desiree Wooldrik, Kim A. M. Lakeman, Bert van het Hof, Joost A. R. Drexhage, Dirk Geerts, Marianna Bugiani, Eleonora Aronica, Reina E. Mebius, Alexandre Prat, Helga E. de Vries, and Arie Reijerkerk J. Neurosci. 2013; 33:1660-1671.
MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression
Juan F. Aranda, Natalia Reglero-Real, Beatriz Marcos-Ramiro, Ana Ruiz-Sáenz, Laura Fernández-Martín, Miguel Bernabé-Rubio, Leonor Kremer, Anne J. Ridley, Isabel Correas, Miguel A. Alonso, and Jaime Millán
Mol. Biol. Cell. 2013; 24:483-494
COM-1/p8 Acts as a Tumour Growth Enhancer in Colorectal Cancer Cell Lines XIN LI, TRACEY A. MARTIN, and WEN G. JIANG Anticancer Res. 2012; 32:1229-1237.
EPLIN-α Expression in Human Oesophageal Cancer and its Impact on Cellular Aggressiveness and Clinical Outcome YINAN LIU, ANDREW J. SANDERS, LIJIAN ZHANG, and WEN G. JIANG Anticancer Res. 2012; 32:1283-1289.
Impact of Yangzheng Xiaoji on the Adhesion and Migration of Human Cancer Cells: the Role of the AKT Signalling Pathway L Ye, K Ji, N Frewer, J Ji, and WG Jiang Anticancer Res. 2012; 32:2537.
Loss of Expression of Growth Differentiation Factor-9 (GDF9) in Human Kidney Cancer and Regulation of Growth and Migration of Kidney Cancer Cells by GDF9 PENG DU, LIN YE, HAN LI, FIONA RUGE, YONG YANG, and WEN G. JIANG Anticancer Res. 2012; 32:4375-4383.
Ezrin, Radixin, and Moesin Are Phosphorylated in Response to 2-Methoxyestradiol and Modulate Endothelial Hyperpermeability (Feb 24) Natalia V. Bogatcheva, Marina A. Zemskova, Boris A. Gorshkov, Kyung Mi Kim, Gregory A. Daglis, Christophe Poirier, and Alexander D. Verin Am. J. Respir. Cell Mol. Biol. 2011; 45:1185-1194.
Bacillus anthracis Lethal Toxin Reduces Human Alveolar Epithelial Barrier Function Marybeth Langer, Elizabeth Stewart Duggan, John Leland Booth, Vineet Indrajit Patel, Ryan A. Zander, Robert Silasi-Mansat, Vijay Ramani, Tibor Zoltan Veres, Frauke Prenzler, Katherina Sewald, Daniel M. Williams, Kenneth Mark Coggeshall, Shanjana Awasthi, Florea Lupu, Dennis Burian, Jimmy Dale Ballard, Armin Braun, and Jordan Patrick Metcalf Infect. Immun. 2012; 80:4374-4387
The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation Jos van Rijssel, Jeffrey Kroon, Mark Hoogenboezem, Floris P. J. van
Alphen, Renske J. de Jong, Elena Kostadinova, Dirk Geerts, Peter L. Hordijk, and Jaap D. van Buul Mol. Biol. Cell. 2012; 23:2831-2844.
Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts Micha Nethe, Bart-Jan de Kreuk, Daniele V. F. Tauriello, Eloise C. Anthony, Barbara Snoek, Thomas Stumpel, Patricia C. Salinas, Madelon M. Maurice, Dirk Geerts, André M. Deelder, Paul J. Hensbergen, and Peter L. Hordijk J. Cell Sci. 2012; 125:3430-3442.
The tyrosine phosphatase SHP2 regulates recovery of endothelial adherens junctions through control of β-catenin phosphorylation Ilse Timmerman, Mark Hoogenboezem, Anton M. Bennett, Dirk Geerts, Peter L. Hordijk, and Jaap D. van Buul Mol. Biol. Cell. 2012; 23:4212-4225.
p120-Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylation Pilar Alcaide, Roberta Martinelli, Gail Newton, Marcie R. Williams,
Alejandro Adam, Peter A. Vincent, and Francis W. Luscinskas Am J Physiol Cell Physiol. 2012; 303:C385-C395.
Circulating Factors Induced by Caloric Restriction in the Nonhuman Primate Macaca Mulatta Activate Angiogenic Processes in Endothelial Cells Anna Csiszar, Danuta Sosnowska, Zsuzsanna Tucsek, Tripti Gautam, Peter Toth, Gyorgy Losonczy, Ricki J. Colman, Richard Weindruch, Rozalyn M. Anderson, William E. Sonntag, and Zoltan Ungvari J Gerontol A Biol Sci Med Sci. published 17 August 2012, 10.1093/gerona/gls158
The atypical chemokine receptor D6 controls macrophage efferocytosis and cytokine secretion during the resolution of inflammation Ester Pashover-Schallinger, Miran Aswad, Sagie Schif-Zuck, Haim Shapiro, Pierre Singer, and Amiram Ariel FASEB J. 2012; 26:3891-3900.
PKCα Activation of p120-Catenin Serine 879 Phospho-Switch Disassembles VE-Cadherin Junctions and Disrupts Vascular Integrity Emily Vandenbroucke St Amant, Mohammad Tauseef, Stephen M. Vogel, Xiao-Pei Gao, Dolly Mehta, Yulia A. Komarova, and Asrar B. Malik Circ. Res. 2012; 111:739-749.
Sustained Adenosine Exposure Causes Lung Endothelial Barrier Dysfunction via Nucleoside transporter-Mediated Signaling Qing Lu, Julie Newton, Vivian Hsiao, Paul Shamirian, Michael R. Blackburn, and Mesias Pedroza Am. J. Respir. Cell Mol. Biol. 2012;47:604-613
Absence of Glial α-Dystrobrevin Causes Abnormalities of the Blood-Brain Barrier and Progressive Brain Edema Chun Fu Lien, Sarajo Kumar Mohanta, Malgorzata Frontczak-Baniewicz, Jerome D. Swinny, Barbara Zablocka, and Dariusz C. Górecki J. Biol. Chem. 2012; 287:41374-41385.
FGF-9 accelerates epithelial invagination for ectodermal organogenesis in real time bioengineered organ manipulation YY Tai, RS Chen, Y Lin, TY Ling, and MH Chen Cell Commun Signal. 2012; 10: 34. [MEDLINE Citation]
Aging-Induced Dysregulation of Dicer1-Dependent MicroRNA Expression Impairs Angiogenic Capacity of Rat Cerebromicrovascular Endothelial Cells Zoltan Ungvari, Zsuzsanna Tucsek, Danuta Sosnowska, Peter Toth, Tripti Gautam, Andrej Podlutsky, Agnes Csiszar, Gyorgy Losonczy, M. Noa Valcarcel-Ares, William E. Sonntag, and Anna Csiszar J Gerontol A Biol Sci Med Sci. published 13 December 2012, 10.1093/gerona/gls242
Sphingosine 1-Phosphate (S1P) Carrier-dependent Regulation of Endothelial Barrier: HIGH DENSITY LIPOPROTEIN (HDL)-S1P PROLONGS ENDOTHELIAL BARRIER ENHANCEMENT AS COMPARED WITH ALBUMIN-S1P VIA EFFECTS ON LEVELS, TRAFFICKING, AND SIGNALING OF S1P1 Brent A. Wilkerson, G. Daniel Grass, Shane B. Wing, W. Scott Argraves, and Kelley M. Argraves J. Biol. Chem. 2012; 287:44645-44653.
High-mobility group box-1 protein activates inflammatory signaling pathway components and disrupts retinal vascular-barrier in the diabetic retina. Mohammad G, Siddiquei MM, Othman A, Al-Shabrawey M, Abu El-Asrar AM. Exp Eye Res. 2012 Dec 21;107C:101-109. doi: 10.1016/j.exer.2012.12.009. [Epub ahead of print] PMID: 23261684
Arresten, a collagen-derived angiogenesis inhibitor, suppresses invasion of squamous cell carcinoma
Aikio M, Alahuhta I, Nurmenniemi S, Suojanen J, Palovuori R, Teppo S, Sorsa T, López-Otín C, Pihlajaniemi T, Salo T, Heljasvaara R, Nyberg P. PLoS One. 2012;7(12):e51044. doi: 10.1371/journal.pone.0051044. Epub 2012 Dec 5.
PMID: 23227231
FGF-9 accelerates epithelial invagination for ectodermal organogenesis in real time bioengineered organ manipulation Tai YY, Chen RS, Lin Y, Ling TY, Chen MH. Cell Commun Signal. 2012 Nov 23;10(1):34. doi: 10.1186/1478-811X-10-34. PMID: 23176204
A vertically aligned carbon nanotube-based impedance sensing biosensor for rapid and high sensitive detection of cancer cells Abdolahad M, Taghinejad M, Taghinejad H, Janmaleki M, Mohajerzadeh S. Lab Chip. 2012 Mar 21;12(6):1183-90. doi: 10.1039/c2lc21028b. Epub 2012 Jan 31. PMID: 22294045
Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation Marimuthu M, Park C, Kim S, Choi CS. Int J Nanomedicine. 2012;7:83-92. doi: 10.2147/IJN.S28465. Epub 2012 Jan 6.
A computational modeling and analysis in cell biological dynamics using electric cell-substrate impedance sensing (ECIS) Chen SW, Yang JM, Yang JH, Yang SJ, Wang JS. Biosens Bioelectron. 2012 Mar 15;33(1):196-203. doi: 10.1016/j.bios.2011.12.052. Epub 2012 Jan 3. PMID: 22261483
Use of cellular electrical impedance sensing to assess in vitro cytotoxicity of anticancer drugs in a human kidney cell nephrotoxicity model Xie F, Xu Y, Wang L, Mitchelson K, Xing W, Cheng J. Analyst. 2012 Mar 21;137(6):1343-50. doi: 10.1039/c2an16141a. Epub 2012 Jan 12. PMID: 22214987
A microfluidic device with passive air-bubble valves for real-time measurement of dose-dependent drug cytotoxicity through impedance sensing Xu Y, Lv Y, Wang L, Xing W, Cheng J. Biosens Bioelectron. 2012 Feb 15;32(1):300-4. doi: 10.1016/j.bios.2011.12.009. Epub 2011 Dec 13. PMID: 22208957
Neuronal markers are expressed in human gliomas and NSE knockdown sensitizes glioblastoma cells to radiotherapy and temozolomide Yan T, Skaftnesmo KO, Leiss L, Sleire L, Wang J, Li X, Enger PŘ. BMC Cancer. 2011 Dec 20;11:524. doi: 10.1186/1471-2407-11-524.
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Have you recently published an article that includes the use of ECIS? If so, submit your publications to Applied BioPhysics via email to Dr. Christian Renken at renken@biophysics.com. We will announce your article in our newsletter, post it on our website and send you
two FREE 8 well arrays! |
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Upcoming Events
Representatives from Applied BioPhysics will be at the following tradeshows and events:
Society of Toxicology
March 10 - 14, 2013
San Antonio, TX
Cleveland Clinic
March 12, 2013
Cleveland, OH
Case Western Reserve University
March 13, 2013
Cleveland, OH
American Association for Cancer Research
April 6 - 10, 2013
Washington, DC
Experimental Biology
April 20 - 24, 2013
Boston, MA
American Thoracic Society International Conference
May 17 - 22, 2013
Philadelphia, PA
10th International Conference on Cerebral Vascular Biology
June 18 - 21, 2013
Montreal, Quebec
Washington University
June 26, 2013
St. Louis, MO
St. Louis University
June 27, 2013
St. Louis, MO
University of Pittsburgh
July 10, 2013
Pittsburgh, PA
IBCA 2013
August 21 - 23, 2013
Budapest, Hungary
NAVBO
Oct. 20 - 24, 2013
Cape Cod, MA
American Society for Cell Biology
Dec. 14 - 18, 2013
New Orleans, LA
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| Tip of the Month:
Adding compounds to study their effect upon ECIS measurements
The ECIS measurement is extremely sensitive; even the responses of cells to small changes in temperature, shear stress, CO2 concentration, etc. are detectable. Because of this, care must be taken when adding compounds to wells to minimize unrelated responses and ensure that the biological effects of the compounds being added are clearly observed.
The following is a suggested protocol:
An hour before the addition of the compound, change the medium over the cells using the medium that will be present when the compound is added - often serum-free medium. This provides the cells time to adjust and equilibrate to the experimental conditions. Follow the equilibration process using ECIS to be certain enough time has elapsed to give a steady impedance signal.
Prepare the compound to be tested at twice the final concentration (2X) in the same solution as that in the wells and allow the solution to equilibrate to incubator conditions. Next, withdraw one-half the volume of medium in a well and then add back this same volume of the 2X solution to achieve the final 1X concentration. The addition should be done slowly, so the cells on the active electrodes are not exposed to shear stress from the fluid flow.
If you are removing the array from the incubator for this addition, be sure to pause the measurement and work quickly to minimize temperature drops. Once the array is returned to the incubator, perform an electrode check to ensure contact has been reestablished and then immediately resume data collection.
If the compound addition is carried out without removing the array from its holder, pausing the measurement during this addition is recommended to avoid any unwanted impedance spikes.
Of course, be sure to always include a control, where all manipulations are carried out but without compound present. |
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WELCOME BACK IVAR & INGER FROM POWDER MOUNTAIN RESORT IN OGDEN, UTAH
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ECIS Humor
Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.
Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!
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