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| The ECIS Connection - November 2012 |
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ECIS Training Schedule
January 17 & 18 2013
Applied BioPhysics is offering a two day in depth ECIS training course. The course will include both lecture and wet lab training. The training will take place at the Applied BioPhysics facility in Troy, NY. Training will be conducted by Dr. Charles Keese, Dr. Ivar Giaever, Dr. Christian Renken and Catherine Toniatti-Yanulavich.
Attendance will be limited to a maximum of 6 people.
Cost is $1,250 per person. This includes two nights of lodging, transportation to and from the hotel to the Applied BioPhysics facility, lunch on both days and dinner Thursday evening. To register, contact Wendy Ladouceur for a registration form at 518-880-6860, ladouceur@biophysics.com or download the form from our website, biophysics.com.
ECIS-related topics to be covered include:
- Experimental design and array selection
- Array preparation and stabilization
- Obtaining good well-to-well and experiment-to-experiment repeatability
- Applying extracellular matrix proteins to the electrodes
- Techniques for array inoculation
- Techniques for addition of compounds to ECIS wells
- Basics of the ECIS software for data acquisition
- Advanced features of the ECIS software for data acquisition
- Basics of the data analysis software
- Advanced features of the data analysis software
- Basics of impedance measurements
- Theory behind ECIS
- Simple and complex impedance and the value of R and C in cell measurements
- Selecting the AC frequency or frequencies for experiments
- Modeling ECIS data
- In situ electroporation
- Cell migration measurements with the ECIS "wound-healing" assay
- Cell migration measurements with the "electric fence"
- A survey of cell biology applications using ECIS
- Fluorescence staining of cells on the ECIS array
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Congratulations to Catherine Toniatti and Mike Yanulavich on their Wedding Day, October 12, 2012
Congratulations to Dr. Ivar Giaever and his wife Inger on their 60th Wedding Anniversary on November 8th.
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ECIS Software
You can find which version you are running by looking at the splash screen on start-up, or by selecting Help | About.
Software Tips:
Latest Software version:
The latest version of the ECIS software is v1.2.104 and the upgrade can be downloaded from:
http://www.biophysics.com/software/ECIS_Software_v1_2_104.msi
If you wish to install the software on a different computer for offline analysis first install the full ECIS package, and then install the update. The full install is available from:
http://www.biophysics.com/software/ECIS_Web_Install.zip
The unzip password is 'NewECISSoftware'. Then run 'Install.bat'.
Experiment Summary:
When running an experiment there is an opportunity to specify an 'Experiment Summary' field that is saved with the dataset. This enables a more detailed description than possible using just the file name.
When running an experiment the Summary can be specified in the GUI just above the Comments region. The Experiment Summary can also be set when the experiment is completed. When loading files using the File Database dialog the Summary field is presented which makes it easy to find the desired dataset.
Setting MFT Frequencies:
When running a multi-frequency time-series (MFT) experiment the frequencies that are collected can be specified by the user from the Acquire | Set Scan Frequencies | Manual menu. This can be used to collect data faster with fewer frequencies, or in more detail with more frequencies.
The frequencies should be sequential from low to high. When the software is loaded the default values are set, so this must be changed first and then will apply to future experiments. The Muti-Freq option on the Data Collection Setup panel indicates the number of frequencies specified when this option has been selected.
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ECIS Webinar Schedule 2012
ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.
All webinars are held at 11:00am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com and scroll to the webinar date of interest. Barrier Function Assays - 11:00 AM EST
November 27, 2012 Real-time Electroporation and Monitoring - 11:00 AM EST December 11, 2012 ECIS Theory - 11:00 AM EST
January 8, 2013 Cell Invasion / Extravasation Assays - 11:00 AM EST
January 22, 2013
Automated Cell Migration - 11:00 AM EST
February 5, 2013
For a more detailed description of each webinar, please visit: http://www.biophysics.com/webinar.php
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| New Publications
Crosstalk Between Reticular Adherens Junctions and Platelet Endothelial Cell Adhesion Molecule-1 Regulates Endothelial Barrier Function. Laura Fernández-Martín, Beatriz Marcos-Ramiro, Carolina L. Bigarella, Mariona Graupera, Robert J. Cain, Natalia Reglero-Real, Anaďs Jiménez, Eva Cernuda-Morollón, Isabel Correas, Susan Cox, Anne J. Ridley, and Jaime Millán
Arterioscler Thromb Vasc Biol. 2012; 32:e90-e102.
Cigarette Smoke Impairs Airway Epithelial Barrier Function and Cell-Cell Contact Recovery.
I.H. Heijink, S.M. Brandenburg, D.S. Postma, and A.J.M. van Oosterhout Eur. Respir. J. 2012; 39:419-428.
The Composition of House Dust Mite is Critical for Mucosal Barrier Dysfunction and Allergic Sensitisation. S Post, M C Nawijn, T L Hackett, M Baranowska, R Gras, A J M van Oosterhout, and I H Heijink Thorax. Published 13 December 2011, 10.1136/thoraxjnl-2011-200606
A Staphylococcus Aureus Pore-Forming Toxin Subverts the Activity of ADAM10 to Cause Lethal Infection in Mice. Inoshima, I. et al., 2011. Nature medicine, 17(10), pp.1310-4.
Protein Kinase C-α and Arginase I Mediate Pneumolysin-Induced Pulmonary Endothelial Hyperpermeability. Rolf Lucas, Guang Yang, Boris A. Gorshkov, Evgeny A. Zemskov, Supriya Sridhar, Nagavedi S. Umapathy, Agnieszka Jezierska-Drutel, Irina B. Alieva, Martin Leustik, Hamid Hossain, Bernhard Fischer, John D. Catravas, Alexander D. Verin, Jean-François Pittet, Ruth B. Caldwell, Timothy J. Mitchell, Stephen D. Cederbaum, David J. Fulton, Michael A. Matthay, Robert W. Caldwell, Maritza J. Romero, and Trinad Chakraborty. Am. J. Respir. Cell Mol. Biol. 2012; 47:445-453.
A Combined Impedance and AlphaLISA-Based Approach to Identify Anti-inflammatory and Barrier-Protective Compounds in Human Endothelium. Maren Pflüger, Aleksandra Kapuscik, Rudolf Lucas, Anita Koppensteiner, Michael Katzlinger, Jouni Jokela, Andreas Eger, Nico Jacobi, Christoph Wiesner, Elisabeth Hofmann, Kamil Önder, Jiri Kopecky, Wolfgang Schütt, and Harald Hundsberger
J Biomol Screen. published 30 August 2012, 10.1177/1087057112458316
Monocytic adhesion molecule expression and monocyte-endothelial cell dysfunction are increased in patients with peripheral vascular disease versus patients with abdominal aortic aneurysms.
ES Lee, EN Van Spyk, KC Chun, RL Pitts, MH Wu, and SY Yuan J Surg Res. 2012.
Non-Invasive Cell Based Impedance Spectroscopy for Real-Time Probing Inhibitory Effects of Graphene Derivatives. B Male, E Lam, J Montes, and JH Luong ACS Appl Mater Interfaces. 2012.
A whole-cell biosensor as in vitro alternative to skin irritation tests.
U Hofmann, S Michaelis, T Winckler, J Wegener, and KH Feller Biosens Bioelectron. 2012. [MEDLINE Citation]
Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions.
SH Huang, SP Lin, CK Liang, and JJ Chen. Biomed Microdevices. 2012. [MEDLINE Citation]
Inhibitory effects of Yangzheng Xiaoji on angiogenesis and the role of the focal adhesion kinase pathway. WG Jiang, L Ye, K Ji, N Frewer, J Ji, and MD Mason Int J Oncol. 2012. [MEDLINE Citation]
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Have you recently published an article that includes the use of ECIS? If so, submit your publications to Applied BioPhysics via email to Dr. Christian Renken at renken@biophysics.com. We will announce your article in our newsletter, post it on our website and send you
two FREE 8 well arrays! |
| Upcoming Events
Representatives from Applied BioPhysics will be at the following tradeshows and events:
December 16 - 18, 2012
San Francisco, CA
Rockefeller University
February 27, 2013
New York, NY
Society of Toxicology
March 10 - 14, 2013
San Antonio, TX
Cleveland Clinic
March 12, 2013
Cleveland, OH
Case Western Reserve University
March 13, 2013
Cleveland, OH
American Association for Cancer Research
April 6 - 10, 2013
Washington, DC
Experimental Biology
April 20 - 24, 2013
Boston, MA
Washington University
June 26, 2013
St. Louis, MO
St. Louis University
June 27, 2013
St. Louis, MO
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| Tip of the Month:
The Importance of High Incubator Humidity for ECIS Measurement
ECIS measurements are affected by the ionic composition of the medium (the electrolyte) bathing the cells. This is not at all surprising as ions are involved in the transfer of electrons at the electrode interface, in characterizing the capacitance of the gold surfaces and in connecting the solution between the electrodes allowing the flow of current.
When proper humidity (commonly 95% or higher relative humidity) is maintained in a cell incubator, there is little evaporation of medium from wells. However, at lower humidity, water will evaporate from the culture wells causing an increase in the medium's ion concentrations. The cells can usually tolerate small changes in ionic strength, but these changes will show up in the impedance measurements. As salt concentration increases, the transfer of electrons at the interface is facilitated, the resistance of the solution decreases and the effective capacitance of the electrodes increases. All of these changes serve to decrease the impedance.
When one runs ECIS experiments and follows the impedance for long periods of time, a gradual impedance drop due to evaporation may incorrectly be interpreted as a loss of the impedance of the cell layer itself.
To avoid this issue, one should always use a good depth of liquid in each well and maintain proper incubator humidity. If one suspects that evaporation is a problem, try increasing the humidity within the incubator space by adding an additional tray of water. The larger surface area of water allows more water molecules to go into the air, increasing the relative humidity and reducing the evaporation problem.
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| ECIS Humor
Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.
Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!
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Article Headline
Know your target audience. Who are your most important customers, clients or prospects, and why? Know what is important to them and address their needs in your newsletter each month. Include a photo to make your newsletter even more appealing. Inserting a link in your article lets you track which topics attract the most interest.
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