Biosensis Focus on miRNA
Dear Doctor,

From purple petunias to the brain and memory; slicing and dicing our way toward understanding post-transcriptional gene expression

 

Purple Petunia

Imagine a better flower, a super purple, purple, superduper purple, petunia. That was the suggestion, the thought and the motivation for geneticist Rich Jorgensen to add an extra purple gene to a purple petunia back in 1986.

 

But what happened in Jorgensen's superduper purple petunia experiments? It failed! - but their final explanation has gone on to change our thinking into how genes are expressed and has provided us with insights into whole new mechanisms of gene control and cellular regulation that may provide new keys for treatments of disease and new ways to engineer existing cells and entire organ systems physiological health.

 

Jorgensen got white, pure white or spotted white flowers instead of purple ones: An unexpected result to say the least since he added extra purple producing genes. Numerous experiments later, it was determined that the RNA itself was interrupting its own expression through complementary binding and the formation of dsRNA molecules; the RNA it seems was literally "interfering with itself" and the concept of RNA interference or RNAi was born.  

 

To read more visit our website... 

Biosensis is pleased to offer excellent new polyclonal antibodies to two key players in the RNA

interference and/or micro-RNA gene regulation pathways:

 

A chicken polyclonal antibody specific for rodent Dicer (C-1557-100) and a pan, anti-mouse eIF2c (Argonaute protein 2) antibody (R-1556-100) capable of staining Ago2 proteins and its isoforms (Ago 1-3). 

Chicken polyclonal antibody to mouse Dicer protein:  
Affinity purified     

 

Biosensis anti-Dicer chicken polyclonal antibody (C-1557-100) recognizes two major bands at 220 kDa (which co-migrated with recombinant Dicer) and a smaller band at 125 kDa. Dicer appears to undergo some fragmentation during processing accounting for the lower molecular weight fragment. The antibody has been shown specific for mouse Dicer in adult mouse brain extracts by western blot, immunoprecipitation and siRNA experiments. This antibody was recently published in J Neurochem. Jul;106(2):650-61.

C-1557-100-WB

 

Western blotting with Chicken polyclonal antibody to

mouse Dicer  protein (C-1557-100) in mouse brain subcellular fractionation. Detection  at 0.03µg/ml.  Protein loaded 10µg.  

 

 

Rabbit polyclonal antibody to mouse elF2c protein: Affinity purified

 

BiosensiR-1556-100 IHC1s antibody against eIF2c protein (R-1556-100) has been used to study the role of small RNA pathways in synaptic functions, particularly in plasticity and learning. Anti-eIF2c antibody has been used to demonstrate that a diverse population of microRNAs is expressed within dendrites and within dendritic spines.

                               

R-1556-100 IHC2

 

Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in (B). Mouse hippocampal area and (D) mouse cerebellar cortex. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1µg/ml overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.     

Over 200 ELISA kits now available!

Biosensis offers you approximately 200 ELISA Kits and 700 highly characterized antibodies that work. To view the full range of kits, visit our website at www.biosensis.com.
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Should your manuscript ever be rejected by ANY journal because of insufficient information about a Biosensis antibody, we will gladly refund TWICE the purchase price of that antibody!

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When you want the best reagents, trust Biosensis 

Between them, Biosensis staff have over 60 years of combined experience in manufacturing and working with antibodies and over 340 peer reviewed research publications in the field of neuroscience. We stand by our guarantee to provide you with the antibody information you need to get your research published.
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