|
Dear Doctor
Are you using an antibody with a high IMPACT FACTOR?
If antibodies had a citation index then anti-NeuN/Fox3 would be at the top of the list - a citation classic - with over 10,000 quality publications to date!
|
|
Why use NeuN/Fox3 antibodies?
Anti NeuN (named for its localization in neuronal nuclei) has been used widely as a reliable tool to detect post-mitotic neurons; important for research in neuroscience, stem cell and developmental biology as well as diagnostic histopathology. Much interest has been stimulated by its identification as Fox3, a member of the family of mammalian homologues of Fox1 (Kim et al, J Biol Chem; 2009). The Fox proteins are about 46kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3. The full length Fox3 was not used as immunogen because the three mammalian homologues, Fox1, 2 and 3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so allowing specific antibodies for each to be generated.
Our range of NeuN/Fox3 antibodies include the following:
Mouse monoclonal antibody to NeuN/Fox3 [Clone 1B7] (1-99): IgG
 Rat brain neural cultures stained with Mouse monoclonal antibody to NeuN/Fox3 [1B7] ( M-377-100) (red), Chicken polyclonal antibody to GFAP ( C-1373-50) (green) and DNA (blue). The Fox3 antibody reveals strong nuclear and distal cytoplasmic staining for NeuN/Fox3 and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This antibody is therefore an excellent marker of neuronal cells. Note that the same immunogen has been used to generate a rabbit polyclonal antibody to NeuN/Fox3 - see product R-3770-100. Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum
Paraformaldehyde fixed frozen section of adult rat cortex stained with Rabbit polyclonal antibody to NeuN/Fox3 ( R-3770-100) in red and counterstained for DNA in blue. Note that this Fox3 antibody stains neuronal nuclei and distal perikarya and does not bind to the nuclei of perikarya of non-neuronal cells and so can be used to identify neurons. |
When you want the best reagents, trust Biosensis
Between them, Biosensis staff have over 60 years of combined experience in manufacturing and working with antibodies and over 340 peer reviewed research publications in the field of neuroscience. We stand by our guarantee to provide you with the antibody information you need to get your research published.
|
