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Dear Doctor
This newsletter highlights the latest additions to our range of neuronal cell markers raised in rabbit and mouse.
When you want the best reagents, trust Biosensis
The staff at Biosensis have many years of combined experience in manufacturing and working with antibodies and have published over 340 peer reviewed research publications in the field of neuroscience. We guarantee to provide you with the antibody information you need to get your research published. |
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Rabbit polyclonal antibody to NeuN/Fox3
 Biosensis is pleased to announce the availability of a new rabbit polyclonal antibody to NeuN/Fox3 R-3770-100. A rabbit host antibody gives a researcher the ability to perform double or triple staining for NeuN in conjunction with antibodies made in mouse, goat, chicken and/ or other species.This antibody was raised against the N-terminal 100 amino acids of human Fox3. It has been used successfully for immunohistochemistry on paraformaldehyde fixed frozen rat brain cortex (adult) tissue and is expected to work in most mammalian species. It does not bind to the nuclei of perikarya of non-neuronal cells making it a good marker to identify neurons. Fox3 is one of a family of mammalian homologues of Fox-1 and is thought to have a role in the regulation of mRNA splicing. Recently Kim et al. (2009) used this antibody to show that NeuN corresponds to Fox3.
Mouse monoclonal antibody to NeuN/Fox3
 Rat brain neural cultures stained with our new Mouse monoclonal antibody to NeuN/Fox3 [1B7] M-377-100 (red), Chicken polyclonal antibody to GFAP C-1373-50 (green) and DNA (blue). The antibody reveals strong nuclear and distal cytoplasmic staining for NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This NeuN antibody is therefore an excellent marker of neuronal cells.
Now you have double and triple neuronal labeling opportunities --- and ONLY FROM Biosensis -- helping you make sense of your research!
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Mouse monoclonal antibody to Nestin
 Antibodies to Nestin are widely used to identify neural stem cells. However Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. Our mouse monoclonal antibody M-1385-100 (4D11) stains both rodent and human Nestin. Image shows mixed cultures of neonatal rat neurons and glia stained with this mouse monoclonal antibody to Nestin M-1385-100.
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Mouse monoclonal antibody to Glial Fibrillary Acidic Protein (GFAP)
 GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. Image shows mixed neuron-glial cultures stained with mouse monoclonal antibody to GFAP [5C10] M-1375-100 (red) and chicken polyclonal antibody to NF-L C-1390-50 (green). This GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA.
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Mouse monoclonal antibody to Peripherin
 The immunogen for this Peripherin monoclonal antibody M-1400-500 [7C5] is full length recombinant rat Peripherin protein expressed in and purified from E.coli. Image shows mixed neuron/glia cultures from newborn rat brain stained with this mouse monoclonal antibody to Peripherin (green) and rabbit polyclonal antibody to NF-L R-1392-50 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both Peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA. This antibody was used recently by Sekerkova et al.(2008). |
