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NanoImaging Services specializes in nanoparticle characterization using transmission electron microscopy. We focus on soft biological materials including antibodies, liposomes, VLPs, viruses and other proteins that are used for vaccines, drug delivery and therapeutics for the Pharmaceutical and Biotechnology industries.
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This is a 3D map of a recombinant Hepatitis B surface antigen particle that was performed at NanoImaging Services in collaboration with Merck Research Laboratories. CryoTEM and single particle methods were used for this characterization. The structure is roughly spherical morphology with ''knuckle''-like protrusions projecting from a smooth surface. Segmentation of the map revealed regions of high density, presumed to be protein, surrounded by regions of lesser density, presumed to be lipid. Map shown end-on for the 4-fold (left) and 2-fold (right). The diameter of the particles is 22nm. The work is described in:
Mulder AM, Carragher B, Towne V, Meng Y, Wang Y, Dieter L, Potter CS, Washabaugh MW, Sitrin RD, Zhao Q. Toolbox for Non-Intrusive Structural and Functional Analysis of Recombinant VLP Based Vaccines: A Case Study with Hepatitis B Vaccine. PLoS ONE. 2012;7(4):e33235.
Recent academic papers that have used similar techniques include:
Lander GC, Baudoux AC, Azam F, Potter CS, Carragher B, Johnson JE. Capsomer Dynamics and Stabilization in the T = 12 Marine Bacteriophage SIO-2 and Its Procapsid Studied by CryoEM. Structure. 2012;20(3):498-503.
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NanoImaging Services will be attending the following upcoming meetings. We would welcome the chance to meet with you.
May 20-25 Vaccine Technology IV, Albufeira, Portugal
NanoImaging Services will participate in the meeting
May 21-23 AAPS Biotech, San Diego, CA
NanoImaging Services will be exhibiting at booth number 208.
July 15-18, Controlled Release Society Annual Meeting, Quebec City, Canada
NanoImaging Services will be exhibiting at booth number 407.
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Contact Information:
NanoImaging Services 11099 North Torrey Pines Rd., #250 San Diego, CA 92037 Ph: 888-675-8261
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