| Welcome to the November issue of NanoImaging Services Inc. newsletter. NanoImaging Services is exclusively focused on reliable, accurate nanoparticle characterization methods using TEM for the pharmaceutical and biotechnology industry. Please take a minute to see how NanoImaging Services can help you and your research. |
HPV virus-like-particles on aluminum containing adjuvant Courtesy of Merck & Co.
|
The yellow arrows point to virus-like particles that are enmeshed in a matrix of an aluminum containing adjuvant. The images were acquired using cryo-transmission electron microscopy (cryoTEM) in which a thin film of the hydrated sample is flash frozen and imaged in the microscope.
NanoImaging Services specializes in using transmission electron microscopy for direct visualization of nanoscale samples in solution, such as: proteins & protein complexes, liposomes & emulsions, viruses & virus like particles, and other nanoscale particles. The company specializes in cryoTEM methods, which preserve samples in their native fully-hydrated state. NanoImaging Services provides critical information to help determine morphology characterization, particle assembly assessment, aggregation states, and 3D structure for your samples. These data are useful throughout all stages of the drug development cycle, including: drug discovery, characterization during scale-up, regulatory review, validation and lot-to-lot comparisons.
|
Recent Papers of Interest:
Cryo-electron tomography of nanoparticle transmigration into liposome.
Le Bihan O, Bonnafous P,
Marak L, Bickel T, Trépout S, Mornet S, De Haas F, Talbot H, Taveau JC, Lambert
O.
J Struct
Biol. 2009
Dec;168(3):419-25.
This paper describes the use of cryo-electron tomography to determine the 3D architecture of of the intermediate steps
of the transport of hydrophilic silica
nanoparticles into large unilamellar neutral DOPC liposomes via an
internalization process.
Structure
of the human Dicer-TRBP complex by electron microscopy.
Structure of the human Dicer-TRBP complex by electron microscopy.
Lau PW, Potter CS, Carragher B, MacRae IJ.
Structure. 2009 Oct
14;17(10):1326-32.
Dicer is a specialized ribonuclease that initiates RNA
interference (RNAi) by cleaving double-stranded RNA (dsRNA) into small RNA
fragments about 22 nucleotides long. The
paper describes the three-dimensional structure of human Dicer bound to the
protein TRBP at approximately 20 A resolution determined by negative-stain
electron microscopy (EM) and single-particle analysis.
|
Contact Information:
NanoImaging Services
10931 North Torrey Pines Rd., #108
San Diego, CA 92037
Ph: 888-675-8261
email: info@nanoimagingservices.com
website: www.nanoimagingservices.com
 |
