Some of this info comes from the CDC and from the TB control person.
1) MMWR From the Quality Control procedure checklist from CDC
Prefilling syringes is not recommended. Tuberculine is absorbed in varying amounts by glass and plastic. To minimize reduction in potency, tuberculine should be administered as soon after the syringe has been filled as possible.
I also spoke to the TB control person and she said each dose needs to be drawn up just before it is given.
Bottom line: Draw it up in front of the patient and give it immediately, no prefilling.
2) MMWR: Tuberculin should be kept refrigerated.
This is from the TB control person: Tuberculin should not be kept in the door but in the main part of the refrigerator, not against the wall or near an air vent. If you are doing a clinic and you do not have a cooler, you can keep a cool pack on the table and place the vial in its box on top of the cool pack. Do not place the cool pack on top of the vial.
Refrigerators need daily temperature checks and need to be kept on a log.
3) MMWR: Tuberculin should be stored in the dark as much as possible.
TB Control person: After you draw up the tuberculine the vial should be returned to its box. It should be stored in the refrigerator in its box.
4) When can a TST be placed if other vaccines are also being administered (e.g., measles, varicella, yellow fever, and smallpox)? A TST should be administered either on the same day as vaccination with live virus or 4--6 weeks later. Vaccines that might cause a false-negative TST result are measles, varicella, yellow fever, smallpox, BCG, mumps, rubella, oral polio, oral typhoid, and live-attenuated influenza. This means you must ask if the have had Flu Mist
5) Should gloves be worn when placing TST? Specific CDC recommendations do not exist regarding this topic. If your local area indicates that universal precautions should be practiced with skin testing, the local areas should determine what precautions should be followed in their setting.
6) If an HCW in a setting has a latex allergy, should this person receive a TST? A person with a latex allergy can receive a TST when latex-free products are used. Latex allergy can be a contraindication to skin testing if the allergy is severe and the products used to perform the test (e.g., syringe plungers, PPD antigen bottle stopper, and gloves) contain latex. Latex-free products are, however, usually available. If a person with a latex allergy does have a TST performed using products or equipment that contain latex, interpretation of the TST results can be difficult, because the TST reaction might be the result of the latex allergy, reaction to PPD, or a combination of both. Consider repeating the TST using latex-free products or use BAMT.
7) Should the TST site be covered with an adhesive bandage? No, avoid covering the TST site with anything that might interfere with reading the TST result (e.g., adhesive bandages, cream, ointment, lotion, liquids, and medication).