Applying The Power of TYPE-C Columns in HPLC
Vol II
In This Issue
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Succinic Acid
Nucleotide Bases
Branch Chain Amino Acid
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Succinic Acid
Succinic
The analysis of succinic acid is mostly done using gas chromatography (GC), enzymatic assays, ion-exclusion chromatography (IEC) or by high performance liquid chromatography (HPLC). However, these methods are time consuming and analysis time is very often longer than 1 hour. In the present application note we are reporting a rapid, cost effective screening method for the detection of this important organic acid. 
  
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In this short email, you can learn more about the great separation power of HPLC using the Cogent Diamond Hydride HPLC columns. 
 
Nucleotides
Leucine & iso Leucine
Succinic Acid
 
Using a mass spectrometer, very little sample prep is needed to fully and completely identify and quantitate molecules that have significant importance in drug development and disease metabolism.
 
Nucleotide Bases
Nucleotide Separation This method is easy to prepare, use and reproduce. Note excellent separation under 100% aqueous conditions with alternate selectivity. These bases are difficult to retain on columns with ordinary silica that contain significant amounts of silanols.
 

Click on the figure to see method conditions. 
Leucine & iso Leucine
BCAA
The addition of weak acetic acid to the mobile phase allows the small difference in the pKas of the two compounds to be used to achieve the resolution for this pair of analytes which have identical m/z values. A few microliters of serum suffices to estimate the leucine and isoleucine present. The two branched-chain amino acids are separated and can be easily quantified.
 
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Bill Ciccone
President 
MicroSolv Technology Corporation