| New Application Notes
Separating Compounds Using Different HPLC Methods |
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Why this email is important: There is a lot of buzz in the world of HPLC and LCMS about the Cogent TYPE-C Silica-Hydride HPLC columns and the novel methods you can use with them; with these columns you can retain hydrophilic and hydrophobic analytes easily and reproducibly. I am sure all chromatographers will be interested in what they can do for them. This announcement shows only two of the problems that are being solved in the real world. |
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| How to Separate Citric and other small
Organic Acids by HPLC |
Column: Cogent Diamond Hydride™, 4µm, 100A Catalog No. 70000-15P-2
Dimensions: 2.1 x 150 mm
Solvents: A: DI water + 0.1% ammonium formate
B: acetonitrile + 0.1% ammonium formate
Mobile Phase Gradient:
Time %B Time %B
0.0 90 6.1 30
2.0 90 7.0 30
5.0 70 7.1 30
6.0 70 10.0 90
Flow Rate: 400 µL/min. Peaks: 1. Maleic acid 115.0031 m/z (M-H)- 2. trans- Aconitic acid 173.0086 m/z (M-H)- 3. Citric acid 191.0192 m/z (M-H)- Sample mixture was prepared in 50% DI water/50% acetonitrile. Detection: ESI - neg - Agilent 6210 MSD TOF mass spectrometer
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| Sodium Benzoate Preservative
by Reverse Phase HPLC |
Column: Cogent Bidentate™C18, 4µm, 100A Catalog No. 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: 90% acetonitrile / 10% DI water / 0.1% ammonium formate
Flow rate: 1.0 mL/minute
Injection Volume: 2 µL
Sample sodium benzoate in fruit juice
t0 = 0.603 min RT = 1.025 min
Detection: UV 254 nm
Click here for more information about this method.
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Valid June 25-October 15, 2008. |
