The ECIS Connection - June 2010           
Links to Articles In This Issue:
Company News - 2010 ECIS Users Meeting
Software Update
ECIS Webinar Schedule
New Publications
Tradeshows 2010
Tip of the Month - Pre-Coating Electrodes with Defined Protein Coats
ECIS Cartoons
Company News

2010 ECIS Users Meeting - August 24-27, 2010

The 2010 ECIS Users Meeting will take place at the Rensselaerville Meeting Center southwest of Albany, NY. The meeting format is a series of twelve 30-minute seminars and fourteen 15-minute platform presentations, as well as poster presentations and workshop sessions.

Seminars will be given by the speakers listed below. Attendees are encouraged to submit abstracts from which the remaining platform talks will be selected. The deadline for abstracts to be considered for platform presentations is July 1, 2010, and those selected will be notified by July 15, 2010. Abstracts are limited to one page of text and one page of figures and will be included in the meeting booklet. Remaining abstracts will be presented as posters.

To register and/or submit abstracts, please contact Christian Renken by email or phone at renken@biophysics.com / 518-880-6860.

Because space is limited we encourage you to register early. We look forward to seeing you in August!

Meeting Speakers:

Dennis Grab
Johns Hopkins University
African trypanosome interactions with the human blood-brain barrier.
Peter Hordijk 
Sanquin Research
The role of the Rac1 GTPase in leukocyte transendothelial migration.

Wen Jiang
Cardiff University
Electric Cell-Substrate Impedance Sensing in the investigation in cancer cell migration and invasion.

Laszlo Kohidai 
Semmelweis University
Cell adhesion induced by Deprenyl and its derivatives - Investigations of adenocarcinoma cell lines (LM2, LM3) by ECIS technique.

Hamed Laroui
Emory University
Targeting drug-loaded nanoparticles to the colon:  A novel therapeutic strategy to treat colitis.

Chun-Min Lo 
University of South Florida
Use of ECIS frequency scan and micromotion measurements to assess low levels of toxins.

John Luong 
National Research Council of Canada
Asrar Malik
University of Illinois at Chicago
Analysis of stem cell function using ECIS.
Chinnaswamy Tirupathi 
University of Illinois at Chicago
Calcium Signaling and Endothelial Barrier Function.

Geerten Van Nieuw Amerongen 
VU University Medical Center
ECIS and vascular permeability.

Joachim Wegener 
University of Regensburg
Intracellular Delivery of Exogeneous Molecules by Electroporation: News and Views from ECIS Readings.

Sandra Wells 
University of Nebraska Medical Center
Lung Serotonin in Asthma Pathophysiology.

To view a copy of the meeting agenda, please click here.

To encourage the attendance of young scientists, ABP will award a $300 travel grant and waive the registration fee for two doctoral students from those submitting abstracts. The awardees' abstracts will also be selected for oral presentation.

The Registration Fee for the meeting is $500 which includes three nights lodging and all meals.  Accommodations are provided for the nights of August 24th, 25th, and 26th. Meal service begins with dinner on the 24th and ends with boxed lunches on the 27th. Wednesday's dinner will be followed by the keynote address from Ivar Giaever, 1973 Nobel Prize winner in Physics, Co-Inventor of ECIS, Professor Emeritus, and CTO of Applied BioPhysics.

The Rensselaerville Meeting Center dates back to 1924 with the Rensselaerville Country Forums. The Center can host up to 84 overnight guests, who are supported by a dedicated staff in an historic country setting, which fosters a spirit of open mindedness and productivity seldom achieved elsewhere.

The latest version of ECIS software is v1.2.42 (for the PC) that includes the following changes:

  • Improved Modeling fit to data, and fixed a number of issues
  • User defined frequency range for MFT scans
  • Faster MFT scans
  • Support for PolyScience water bath
  • Support for New Brunswick Galaxy 14S incubator
  • Added temperature and CO2 data collection for above instruments
  • Allow grouping of normalized data
  • Change number of error bars displayed with grouping
If your system is connected to the internet, go to 'Help | Check for Updates' in the software.

You can also install ECIS software on different computers for offline analysis using the CD that came with your system or contact info@biophysics.com for details.

A new version of the EdgePort USB driver has also been released (v5.30).  The driver can be downloaded from:

To install the new driver, run the downloaded file and extract the files to 'c:\EdgePort Drivers'.  Then open the 'EdgePort Configuration Utility', go to the 'Advanced' tab, click 'Uninstall', and then reboot the PC.  When the new hardware is detected, manually specify the driver directory as 'c:\EdgePort Drivers'.  You may need to do this multiple times as there are multiple COM ports.

ECIS Webinar Schedule 2010

ECIS application webinars review the topics listed below in 20 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.

All webinars are held at 11:00am EST. To register for a webinar, please go to:
  • Cell Attachment and Spreading - June 15, 2010
  • Signal Transduction Assays - June 22, 2010
  • Toxicology with ECIS - July 6, 2010
  • ECIS Theory  - July 20, 2010
  • Cell Invasion / Extravasation Assays - September 7, 2010
  • Automated Cell Migration - September 21, 2010
  • Barrier Function Assays - October 5, 2010
  • Real-time Electroporation and Monitoring - October 19, 2010
  • Cell Attachment and Spreading Measurements - November 2, 2010
  • Signal Transduction Assays - November 16, 2010
  • Toxicology with ECIS - December 7, 2010
For a more detailed description of each webinar, please visit: http://www.appliedbiophysics.com/contactUs/webinar.html.
New Publications

Our Application Scientist, Christian Renken, contributed to the following publication from researchers at the University of Washington School of Medicine and San Diego State University:

Regulation of CovR expression in Group B streptococcus impacts blood-brain barrier penetration. A Lembo, MA Gurney, K Burnside, A Banerjee, M de Los Reyes, JE Connelly, WJ Lin, KA Jewell, A Vo, CW Renken, KS Doran, and L Rajagopal. Mol Microbiol. 2010. 

Development of an electrode cell impedance method to measure osteoblast cell activity in magnesium-conditioned media. Y Yun, Z Dong, Z Tan, and MJ Schulz Anal Bioanal Chem. 2010. Volume: 396   Issue: 8   Pages: 3009-3015.

Real-Time Electrical Impedance Detection of Cellular Activities of Oral Cancer Cells.
LR Arias, CA Perry, and L Yang. Biosens Bioelectron. 2010. 

The PI3K p110α isoform regulates endothelial adherens junctions via Pyk2 and Rac1
RJ Cain, B Vanhaesebroeck, and AJ Ridley. J Cell Biol. 2010; vol:188 iss:6 pg:863 -876.
EGFR signaling contributes to house dust mite-induced epithelial barrier dysfunction
I.H. Heijink, A. van Oosterhout, and A. Kapus. Eur. Respir. J. published 29 March 2010, 10.1183/09031936.00125809  [Abstract].

Characterization of cell adhesion in airway epithelial cell types using electric cell-substrate impedance sensing. I. H. Heijink, S. M. Brandenburg, J. A. Noordhoek, D. S. Postma, D-J. Slebos, and A. J. M. van Oosterhout Eur. Respir. J. 2010; 35:894-903. 

Cellular mechanisms of IL-17-induced blood-brain barrier disruption. Jula Huppert, Dorothea Closhen, Andrew Croxford, Robin White, Paulina Kulig, Eweline Pietrowski, Ingo Bechmann, Burkhard Becher, Heiko J. Luhmann, Ari Waisman, and Christoph R. W. Kuhlmann

ADAM15 regulates endothelial permeability and neutrophil migration via Src/ERK1/2 signaling Chongxiu Sun, Mack H. Wu, Mingzhang Guo, Mark L. Day, Eugene S. Lee, and Sarah Y. Yuan. Cardiovasc Res. published 10 April 2010, 10.1093/cvr/cvq060

Effects of (-)-epigallocatechin gallate on RPE cell migration and adhesion. CM Chan, JH Huang, HS Chiang, WB Wu, HH Lin, JY Hong, and CF Hung Mol Vis. 2010; 16: 586. 

Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity
Nicolas Schlegel, Michael Meir, Wolfgang-Moritz Heupel, Bastian Holthöfer, Rudolf E. Leube, and Jens Waschke. Am J Physiol Gastrointest Liver Physiol. 2010; 298:G774-G783. 

Calcium/calmodulin-dependent protein kinase II delta6 (CaMKIIdelta6) and RhoA involvement in thrombin-induced endothelial barrier dysfunction. Zhen Wang, Roman Ginnan, Iskandar F. Abdullaev, Mohamed Trebak, Peter A. Vincent, and Harold A. Singer. J. Biol. Chem. published 4 May 2010, 10.1074/jbc.M110.120790 

Effects of Negative Pressures on Epithelial Tight Junctions and Migration in Wound Healing
Chih-Chin Hsu, Wen Chung Tsai, Carl Pai-Chu Chen, Yun-Mei Lu, and Jong-Shyan Wang Am J Physiol Cell Physiol. published 5 May 2010, 10.1152/ajpcell.00504.2009

Group V Phospholipase A2 Mediates Barrier Disruption of Human Pulmonary Endothelial Cells Caused by LPS in Vitro. Steven M. Dudek, Nilda M. Muñoz, Anjali Desai, Christopher M. Osan, Angelo Y. Meliton, and Alan R. Leff. Am. J. Respir. Cell Mol. Biol. published 6 May 2010, 10.1165/rcmb.2009-0446OC

Adenosine protected against pulmonary edema through transporter- and receptor A2-mediated endothelial barrier enhancement. Qing Lu, Elizabeth O. Harrington, Julie Newton, Brian Casserly, Gregory Radin, Rod Warburton, Yang Zhou, Michael R. Blackburn, and Sharon Rounds. Am J Physiol Lung Cell Mol Physiol. 2010; 298:L755-L767. 

Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis. M Tarantola, AK Marel, E Sunnick, H Adam, J Wegener, and A Janshoff. Integr Biol (Camb). 2010; 2: 139. 

Antipermeability Function of PEDF Involves Blockade of the MAP Kinase/GSK/β-Catenin Signaling Pathway and uPAR Expression. Jinling Yang, Elia J. Duh, Ruth B. Caldwell, and M. Ali Behzadian Invest. Ophthalmol. Vis. Sci. 2010; 51:3273-3280. 

Focal-adhesion targeting links caveolin-1 to a Rac1-degradation pathway
Micha Nethe, Eloise C. Anthony, Mar Fernandez-Borja, Rob Dee, Dirk Geerts, Paul J. Hensbergen, André M. Deelder, Gudula Schmidt, and Peter L. Hordijk. J. Cell Sci. 2010; 123:1948-1958. 

Claudin-2, a component of the tight junction, forms a paracellular water channel.
Rita Rosenthal, Susanne Milatz, Susanne M. Krug, Beibei Oelrich, Jörg-Dieter Schulzke, Salah Amasheh, Dorothee Günzel, and Michael Fromm. J. Cell Sci. 2010; 123:1913-1921

Zeaxanthin inhibits PDGF-BB-induced migration in human dermal fibroblasts.
NL Wu, YC Chiang, CC Huang, JY Fang, DF Chen, and CF Hung. Exp Dermatol. 2010. 

ALCAM, Activated Leukocyte Cell Adhesion Molecule, Influences the Aggressive Nature of Breast Cancer Cells, a Potential Connection to Bone Metastasis. SIMON DAVIES and WEN G. JIANG. Anticancer Res. 2010; 30:1163-1168. 

Have you recently published an article that includes the use of ECIS?
If so, submit your publications to Applied BioPhysics via email to Nancy Vlahos at vlahos@biophysics.com. We will announce your article in our newsletter, post it on our website and send you 2 FREE 8 well arrays!

Visit Us at Upcoming Events

Applied BioPhysics will have ECIS demonstrations and informational literature at the following tradeshows and events: 

16th International Vascular Biology Meeting
June 20 - 24, 2010
Los Angeles, CA

2010 ECIS Users Meeting
August 24 - 27, 2010
Rensselaerville, NY

American Society for Cell Biology
ASCB 50th Annual Meeting
December 11-15, 2010
Philadelphia, PA

Tip of the Month:

Pre-Coating Electrodes with Defined Protein Coats

ECIS electrodes shipped from Applied BioPhysics have no macromolecular coatings. When culture medium is added to the arrays proteins and other large molecules in the medium immediately adsorb to the very wettable gold surface as they do with any uncoated tissue culture dish.  

Often it is desirable to alter cell behavior by pre-coating the electrodes with fibronectin, laminin or other extracellular matrix proteins. To do so we suggest using the following protocol:

Prepare a solution of the desired protein at 100 micrograms per ml or more in 0.15M NaCl.  We have found that the use of phosphate buffer (PBS) can seriously interfere with the adsorption of some proteins, so if a buffer is required, a mild Tris solution (e.g. 0.01M) is recommended.  The electrode arrays are stable under acidic conditions, so when coating with collagen there is no problem using solutions containing acetic acid.
To coat the electrode, place the protein solution in the bottom of the well for 10 minutes or more.  If the protein is valuable, lower protein concentrations can be used with increased times for adsorption.  In addition, when using a 1E array it is only necessary to coat the small active electrode (250 micrometer diameter).  Just a few microliters can be carefully applied to this small spot, as only cells upon the active electrode are observed in the ECIS measurement. 
Once adsorption has taken place, an approximate mono-molecular layer of the protein will coat the surface.  You can safely rinse the protein solution from the well with sterile medium, saline or water without concern of removing the adsorbed layer.  Medium can now be added and inoculation begun. 
Note: Drying protein solutions in place may damage the electrodes.

ECIS Humor

Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.

Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!

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