Company News
2010 ECIS Users Meeting - August 24-27, 2010
The 2010 ECIS Users
Meeting will take place at the Rensselaerville Meeting
Center southwest of Albany, NY. The meeting format is a
series of twelve 30-minute seminars and fourteen 15-minute platform
presentations, as well as poster presentations and workshop sessions.
Seminars will be given by the speakers listed below. Attendees are encouraged to submit abstracts from which the remaining
platform talks will be selected. The deadline for abstracts to be
considered for platform presentations is July 1, 2010, and those
selected will be notified by July 15, 2010. Abstracts are
limited to one page of text and one page of figures and will be included
in the meeting booklet. Remaining abstracts will be presented as posters.
To register and/or submit abstracts, please
contact Christian Renken by email or phone at renken@biophysics.com /
518-880-6860.
Because space is limited we encourage you to register early. We look forward to seeing you in August!
Meeting Speakers:
Dennis Grab
Johns Hopkins
University
African trypanosome interactions with the human
blood-brain barrier.
Peter Hordijk
Sanquin Research
The role of the Rac1 GTPase in leukocyte
transendothelial migration.
Wen Jiang
Cardiff
University
Electric Cell-Substrate Impedance Sensing in
the investigation in cancer cell migration and invasion.
Laszlo Kohidai
Semmelweis University
Cell adhesion induced by Deprenyl and its derivatives - Investigations
of adenocarcinoma cell lines (LM2, LM3) by ECIS technique.
Hamed Laroui
Emory University
Targeting
drug-loaded nanoparticles to the colon: A
novel therapeutic strategy to treat colitis.
Chun-Min Lo
University of South
Florida
Use
of ECIS frequency scan and micromotion measurements to assess low levels
of toxins.
John Luong
National Research Council of Canada
Asrar Malik
University of Illinois at Chicago
Analysis of stem cell function using ECIS.
Chinnaswamy Tirupathi
University of Illinois at Chicago
Calcium
Signaling and Endothelial Barrier Function.
Geerten Van Nieuw Amerongen
VU
University Medical Center
ECIS and
vascular permeability.
Joachim Wegener
University of Regensburg
Intracellular Delivery of Exogeneous Molecules
by Electroporation: News and Views from ECIS Readings.
Sandra Wells
University of Nebraska
Medical Center
Lung
Serotonin in Asthma Pathophysiology.
To view a copy of the meeting agenda, please click here.
To encourage the
attendance of young scientists, ABP will award a $300 travel grant and
waive the registration fee for two doctoral students from those
submitting abstracts. The awardees' abstracts will also be selected for
oral presentation.
The Registration Fee for the meeting is $500 which includes three
nights lodging and all meals. Accommodations are provided for the
nights of August 24th, 25th, and 26th. Meal service begins with dinner
on the 24th and ends with boxed lunches on the 27th. Wednesday's
dinner will be followed by the keynote address from Ivar Giaever, 1973
Nobel Prize winner in Physics, Co-Inventor of ECIS, Professor Emeritus,
and CTO of Applied BioPhysics.
The
Rensselaerville Meeting Center dates back to 1924 with
the Rensselaerville Country Forums. The Center can host up to 84
overnight guests, who are supported by a dedicated staff in an historic
country setting, which fosters a spirit of open mindedness and
productivity seldom achieved elsewhere.
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Software
The latest version of ECIS software is v1.2.42 (for the
PC) that includes the following changes:
- Improved
Modeling fit to data, and fixed a number of issues
-
User
defined frequency range for MFT scans
-
Faster
MFT scans
-
Support
for PolyScience water bath
-
Support
for New Brunswick Galaxy 14S incubator
-
Added
temperature and CO2 data collection for above instruments
-
Allow
grouping of normalized data
-
Change
number of error bars displayed with grouping
http://www.biophysics.com/software/ECIS_Software_Update.msi
If your system is connected to the internet, go to 'Help | Check
for Updates' in the software.
You can also install ECIS software on different computers
for offline analysis using the CD that came with your system or contact info@biophysics.com for details.
A new version of the EdgePort USB driver has also been
released (v5.30). The driver can be
downloaded from:
To install the new driver, run the downloaded
file and extract the files to 'c:\EdgePort Drivers'. Then open the 'EdgePort Configuration
Utility', go to the 'Advanced' tab, click 'Uninstall', and then reboot the
PC. When the new hardware is detected,
manually specify the driver directory as 'c:\EdgePort Drivers'. You may need to do this multiple times as
there are multiple COM ports.
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ECIS Webinar Schedule 2010
ECIS application webinars review the topics listed below in 20 minute, web-based, interactive seminars presented by Applied BioPhysics president and co-founder, Dr. Charles Keese.
All webinars are held at 11:00am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com
- Cell Attachment and Spreading - June 15, 2010
- Signal Transduction Assays - June 22, 2010
-
Toxicology with ECIS - July 6, 2010
-
ECIS Theory - July 20, 2010
-
Cell Invasion / Extravasation Assays - September 7,
2010
-
Automated Cell Migration - September 21, 2010
-
Barrier Function Assays - October 5, 2010
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Real-time Electroporation and Monitoring - October
19, 2010
-
Cell Attachment and Spreading Measurements - November
2, 2010
-
Signal Transduction Assays - November 16, 2010
-
Toxicology with ECIS - December 7, 2010
For a more detailed description of each webinar, please visit: http://www.appliedbiophysics.com/contactUs/webinar.html.
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New Publications
Our Application Scientist, Christian
Renken, contributed to the following publication from researchers at the University of Washington School of Medicine and San Diego State University:
Regulation of CovR expression in Group B streptococcus impacts blood-brain barrier penetration. A Lembo, MA Gurney, K
Burnside, A Banerjee, M de Los Reyes, JE Connelly, WJ
Lin, KA Jewell, A Vo, CW Renken, KS Doran, and L Rajagopal. Mol
Microbiol.
2010.
Development of an electrode cell impedance method to measure osteoblast cell activity in magnesium-conditioned media. Y Yun, Z Dong, Z Tan, and MJ Schulz Anal Bioanal Chem.
2010. Volume: 396 Issue: 8 Pages: 3009-3015.
Real-Time Electrical Impedance Detection of Cellular Activities of Oral Cancer Cells.
LR
Arias, CA Perry, and L Yang. Biosens Bioelectron.
2010.
The PI3K p110α isoform regulates endothelial adherens junctions via Pyk2 and Rac1
RJ Cain, B Vanhaesebroeck, and AJ
Ridley. J
Cell Biol. 2010; vol:188 iss:6 pg:863 -876.
EGFR signaling contributes to house dust mite-induced epithelial barrier dysfunction
I.H. Heijink, A. van Oosterhout, and A. Kapus. Eur. Respir. J.
published 29 March 2010, 10.1183/09031936.00125809 [Abstract].
Characterization of cell adhesion in airway epithelial cell types using electric cell-substrate impedance sensing. I. H. Heijink, S. M. Brandenburg, J. A. Noordhoek, D. S. Postma, D-J. Slebos,
and A. J. M. van Oosterhout Eur. Respir. J.
2010; 35:894-903.
Cellular mechanisms of IL-17-induced blood-brain barrier disruption. Jula Huppert, Dorothea Closhen, Andrew Croxford, Robin White, Paulina Kulig,
Eweline Pietrowski, Ingo Bechmann, Burkhard Becher, Heiko J. Luhmann, Ari
Waisman, and Christoph R. W. Kuhlmann
ADAM15 regulates endothelial permeability and neutrophil migration via Src/ERK1/2 signaling Chongxiu Sun, Mack H. Wu, Mingzhang Guo, Mark L. Day, Eugene S. Lee, and Sarah
Y. Yuan. Cardiovasc Res.
published 10 April 2010, 10.1093/cvr/cvq060
Effects of (-)-epigallocatechin gallate on RPE cell migration and adhesion. CM Chan, JH Huang, HS Chiang, WB Wu, HH Lin, JY Hong, and CF Hung Mol Vis. 2010;
16: 586.
Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity
Nicolas Schlegel, Michael Meir, Wolfgang-Moritz Heupel, Bastian Holthöfer,
Rudolf E. Leube, and Jens Waschke. Am J Physiol Gastrointest
Liver Physiol. 2010; 298:G774-G783.
Calcium/calmodulin-dependent protein kinase II delta6 (CaMKIIdelta6) and RhoA involvement in thrombin-induced endothelial barrier dysfunction. Zhen Wang, Roman Ginnan, Iskandar F. Abdullaev, Mohamed Trebak, Peter A.
Vincent, and Harold A. Singer.
J. Biol. Chem. published 4 May 2010, 10.1074/jbc.M110.120790
Effects of Negative Pressures on Epithelial Tight Junctions and Migration in Wound Healing
Chih-Chin Hsu, Wen Chung Tsai, Carl Pai-Chu Chen, Yun-Mei Lu, and Jong-Shyan
Wang Am J Physiol Cell Physiol.
published 5 May 2010, 10.1152/ajpcell.00504.2009
Group V Phospholipase A2 Mediates Barrier Disruption of Human Pulmonary Endothelial Cells Caused by LPS in Vitro. Steven M. Dudek, Nilda M. Muñoz, Anjali Desai, Christopher M. Osan, Angelo Y.
Meliton, and Alan R. Leff. Am. J. Respir. Cell Mol.
Biol. published 6 May 2010, 10.1165/rcmb.2009-0446OC
Adenosine protected against pulmonary edema through transporter- and receptor A2-mediated endothelial barrier enhancement. Qing Lu, Elizabeth O. Harrington, Julie Newton, Brian Casserly, Gregory Radin,
Rod Warburton, Yang Zhou, Michael R. Blackburn, and Sharon Rounds. Am J Physiol Lung Cell Mol
Physiol. 2010; 298:L755-L767.
Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis. M Tarantola, AK Marel, E Sunnick, H Adam, J Wegener, and A Janshoff. Integr Biol (Camb).
2010; 2: 139.
Antipermeability Function of PEDF Involves Blockade of the MAP Kinase/GSK/β-Catenin Signaling Pathway and uPAR Expression. Jinling Yang, Elia J. Duh, Ruth B. Caldwell, and M. Ali Behzadian Invest. Ophthalmol. Vis.
Sci. 2010; 51:3273-3280.
Focal-adhesion targeting links caveolin-1 to a Rac1-degradation pathway
Micha Nethe, Eloise C. Anthony, Mar Fernandez-Borja, Rob Dee, Dirk Geerts, Paul
J. Hensbergen, André M. Deelder, Gudula Schmidt, and Peter L. Hordijk. J. Cell Sci.
2010; 123:1948-1958.
Claudin-2, a component of the tight junction, forms a paracellular water channel.
Rita Rosenthal, Susanne Milatz, Susanne M. Krug, Beibei Oelrich, Jörg-Dieter
Schulzke, Salah Amasheh, Dorothee Günzel, and Michael Fromm. J. Cell Sci.
2010; 123:1913-1921
Zeaxanthin inhibits PDGF-BB-induced migration in human dermal fibroblasts.
NL Wu, YC Chiang, CC Huang, JY Fang, DF Chen, and CF Hung. Exp Dermatol.
2010.
ALCAM, Activated Leukocyte Cell Adhesion Molecule, Influences the Aggressive Nature of Breast Cancer Cells, a Potential Connection to Bone Metastasis. SIMON DAVIES and WEN G. JIANG. Anticancer Res.
2010; 30:1163-1168.
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Have you recently published an article that includes the use of ECIS? If so, submit your publications to Applied BioPhysics via email to Nancy Vlahos at vlahos@biophysics.com. We
will announce your article in our newsletter, post it on our website and send you 2
FREE 8 well arrays!
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Tip of the Month:
Pre-Coating Electrodes with Defined Protein Coats
ECIS electrodes shipped from Applied BioPhysics have no macromolecular
coatings. When culture medium is added
to the arrays proteins and other large molecules in the medium
immediately adsorb to the very wettable gold surface as they do with any
uncoated tissue culture dish.
Often it
is desirable to alter cell behavior by pre-coating the electrodes with
fibronectin, laminin or other extracellular matrix proteins. To do so we suggest using the following protocol:
Prepare a solution of the desired protein at 100 micrograms per ml or more in 0.15M
NaCl. We have found that the use of
phosphate buffer (PBS) can seriously interfere with the adsorption of some
proteins, so if a buffer is required, a mild Tris solution (e.g. 0.01M) is
recommended. The electrode arrays are
stable under acidic conditions, so when coating with collagen there is no
problem using solutions containing acetic acid.
To
coat the electrode, place the protein solution in the bottom of the well
for 10 minutes or more. If the protein
is valuable, lower protein concentrations can be used with increased times for
adsorption. In addition, when using a
1E array it is only necessary to coat the small active electrode (250
micrometer diameter). Just a few
microliters can be carefully applied to this small spot, as only cells upon the
active electrode are observed in the ECIS measurement.
Once
adsorption has taken place, an approximate mono-molecular layer of the protein
will coat the surface. You can
safely rinse the protein solution from the well with sterile medium, saline or
water without concern of removing the adsorbed layer. Medium can now be added and inoculation
begun.
Note:
Drying protein solutions in place may damage the electrodes.
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| ECIS Humor
Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.
Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!
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