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Practical Methods for In Vitro Toxicology Workshop  

Sample Agenda

Day One (half day)



  * Orientation, Introduction of Staff and Participants, and Roundtable Goals

  * Course Introduction and Overview of In Vitro Toxicology

  * Introduction to the Good Laboratory Practices: Emphasis on Application in the Laboratory

  * Judging Laboratory Quality: The Importance of Controls, Benchmarks, Training and Supplier  


  * Political/Regulatory Climate for In Vitro Toxicology and Impacts Upon Testing Requirements 

Day Two


 * Overview of In Vitro Ocular Testing Methods

* Development of the Bovine Cornea Opacity and Permeability (BCOP) Assay

* 3-D Ocular Tissue Models


 * Multiple Uses of Basic Cytotoxicity Studies

        * Determination of the Appropriate Assay and End Points

        * Interpretation of Resulting Data from Cytotoxicity Studies  

* Oxidative Stress Endpoints


 * Introduction to In Vitro Dermal Irritation Testing

* Monolayer Culture Systems

* 3D Tissue Models



 * Pipettor Review
 * Dermal Alternatives Laboratory
 * Phototoxicity Laboratory

Day Three


  * In Vitro Methods for Screening Skin Sensitizers

  * Embryo Toxicity with Focus on the Embryonic Stem Cell Toxicity (EST) Assay

  * Hepatocyte Culture Systems in #-D Matrices

  * Micronucleus Assay for Topically-Applied Ingredients in the EpiDerm Tissue Construct

  * Long-Term Corneal Culture (LTCC) - an In Vitro Recovery Model



  * Phototoxicity Assay continued 



  * Use of Histological Evaluation as a Complementary Endpoint in the BCOP Assay

  * Concepts for In Vitro Recovery Model Design



  * Phototoxicity Assay continued

  * Ocular Alternatives

  * Demonstration of Cytosensor Microphysiometer

  * Demonstration of EST Assay

  * Demonstration of LTCC

  * Demonstration of Micronucleus Assay 

Day Four


  * In Vitro Testing for Vaginal Irritation Assessment

  * In Vitro Percutaneous Absorption - Skin Penetration



  * Dermal Alternatives Assay continued

  * Phototoxicity Assay continued

  * Demonstration of Skin Penetration

  * Dermal Alternatives Assay conclusion

  * Demonstration of Corrositex Assay 

  * Demonstration of Corrosivity in 3-D Tissue Constructs



  * Discuss Calculation of Data for Laboratory Assays

  * Course Summary, Open Discussion, Completion of Evaluations, Presentation of Certificates